Chemical exchange saturation transfer (CEST) imaging can provide information from metabolites, lipids and proteins, which are not directly detectable with conventional MRI sequences due to their low concentration in tissue. CEST employs a frequency-selective radio-frequency saturation to saturate magnetization of protons from the solute pool. CEST contrast originates from the chemical exchange of labile protons from solutes with those from water molecules. Saturation of the solute pool results in a detectable signal attenuation of the water pool depending on the exchange rate and the solute concentration. This effect can be used to indirectly observe changes in metabolite concentration or protein content, as well as in tissue parameters such as intracellular pH. Compared to MR spectroscopy, CEST can provide localized images with higher spatial resolution. This makes CEST very advantageous for clinical applications.