The baseline data collection of the NEO study took place between September 2008 and October 2012. In total, 6,671 men and women participated in the NEO study. Participants completed questionnaires at home and visited the NEO study center for an extensive physical examination.
Participants were invited to visit the NEO study center at the LUMC after an overnight fast. Prior to the study visit, participants collected their urine over 24 hours and completed questionnaires at home. The participants were asked to bring all medication they were using in the month preceding the study visit to the NEO study site. This included prescription medication, as well as over-the-counter drugs such as painkillers.
At the NEO study center several measurements have been performed, including an extensive physical examination and blood sampling. In addition, in random subsets of participants we assessed abdominal fat, aortic pulse wave velocity, cardiac function, knee osteoarthritis and brain structure by magnetic resonance imaging (MRI), in addition to hepatic triglyceride content measurements by 1H-magnetic resonance spectroscopy (MRS), indirect calorimetry, dual-energy X-ray absorptiometry (DXA-scan), actigraphy, accelerometry, heart rate variability measurements, and sidestream darkfield (SDF) imaging of the glycocalyx.
For more detailed information about the NEO study design and methods: de Mutsert R, den Heijer M, Rabelink TJ, Smit JW, Romijn JA, Jukema JW, de Roos A, Cobbaert CM, Kloppenburg M, le Cessie S, Middeldorp S, Rosendaal FR. The Netherlands Epidemiology of Obesity (NEO) study: study design and data collection. Eur J Epidemiol 2013;28:513-523.
PDF documents of the questionnaires used for the NEO baseline data collection can be found under Baseline questionnaires.