We are looking for a motivated PhD candidate for the project titled ‘Hitting the sweet spot of dendritic cells and macrophages: Targeting O-GlcNAcylation to shape Type 2 immune responses’.
• You will contribute to research on type 2 immune responses
• This research focuses on the role of a specific form of glycosylation in dendritic cells and macrophages
• You will use several in vivo models and clinical material
• This is a multidisciplinary project in an academic setting
What you do
Type 2 immune responses offer protection against parasitic worm infections, but are also driving allergic responses and are associated with fostering tumor growth. Therefore, there is great interest in understanding how type 2 immune responses are initiated and regulated. As part of the highly promising field of immunemetabolism, this research focuses on the role of a particular type of intracellular glycosylation, termed O-GlcNAcylation, in dendritic cells (DCs) and macrophages (Mphs) in shaping type 2 immune responses. To this end, both in vitro DC and Mph models, in vivo models and clinical material will be used. The project comprises of fundamental molecular and cellular work as well as more translational research, and involves transcriptomics, mass spectrometry and mass cytometry. This project ultimately aims to define whether O-GlcNAcylation in DCs and Mphs could be targeted to shape Type 2 immune responses for therapeutic gain.
What we ask
You have a master’s degree in biomedical sciences, preferably with experience in cellular immunology and/or metabolism. Practical experience with cell culture and mice would be an asset. Additional experiences with molecular and cell biology tools (Western Blot and mass spectrometry) will be considered an advantage. You are a highly motivated independent team player with a flexible work approach and with the ability to work effectively within a multidisciplinary team. English language skills are essential for communication within the team, but also for scientific presentations and publications.
You will be employed on the basis of a 36-hour week. Appointment is for a maximum of four years, to be completed with a doctoral thesis. Your salary is € 2,422 gross per month in the first year, amounting to € 3,103 in the fourth year (scale PhD students, cao UMC).
References will be requested.
Hine van Os
You will work primarily within the Leiden Immunoparasitology Group (LIPG), which is part of the Department of Parasitology. While a main research line of the department is centred around understanding the immunological basis of host parasite interactions, a new research line within the department focuses on cellular immunemetabolism and metabolic diseases. In addition, given the multidisciplinary nature of this project, you will perform this research in close collaboration with other departments, in particular the Center for Proteomics and Metabolomics. This ensures that this research will be performed in a very stimulating and diverse environment with an excellent embedding of the project within the LUMC.
Your career at the LUMC
The LUMC offers opportunities to maintain and develop your knowledge and skills. We offer internal courses and in-service training to help you with your personal development. We also offer services for mobility and career advice.