Metabolomics

Metabolomics is defined as the systemic analysis of the metabolome, which encompasses the totality of the metabolites in a biological system, i.e., the end-points and intermediate products of metabolism. Metabolomics has become a powerful diagnostic and prognostic tool to investigate, for example, susceptibility to disease, disease mechanisms and treatment outcome.

The metabolomics group houses several state-of-the art mass spectrometers ranging from triple-quadrupole to quadrupole time-of-flight mass analyzers. Our work focuses on the development and application of advanced analysis techniques for small molecules in the field of metabolomics. As main analytical techniques we use liquid chromatography (LC), including reversed-phase and hydrophilic interaction chromatography, and gas chromatography (GC) in combination with mass spectrometry as well as nuclear magnetic resonance spectroscopy (NMR) and advanced data analysis approaches. Next to global metabolic profiling (untargeted metabolomics), special emphasis is put on quantitative analysis using isotope-dilution approaches. Our state-of-the art analytical platforms are applied in collaboration with several groups (within and outside the LUMC) in a variety of research fields, such as inflammation, infection, obesity and migraine. Our main interests include lipid remodelling during acute inflammation, the effects of bioactive lipids on the course of inflammation, metabolic profiling in the field of biomarker discovery, metabolic changes during infection as well as the relation between altered metabolite profiles, inflammation, infection and disease.


Contact

Dr. Martin Giera
Phone: +31 715269527


Staff

Dr. Martin Giera
Dr. Oleg A. Mayboroda
Dr. Aswin Verhoeven
Dr. Rico Derks
Hulda Jonasdottir, Msc.
Dr. Sarantos Kostidis
Ing. Evelyne Steenvoorden

Marieke Heijink, MSc.


Key publications

  1. Schlegel M, Köhler D, Körner A, Granja T, Straub A, Giera M, Mirakaj V. The neuroimmune guidance cue netrin-1 controls resolution programs and promotes liver regeneration. Hepatology. 2015 Nov 17. doi: 10.1002/hep.28347.

  2. Jónasdóttir HS, Papan C, Fabritz S, Balas L, Durand T, Hardardottir I, Freysdottir J, Giera M. Differential mobility separation of leukotrienes and protectins. Anal Chem. 2015 May 19;87(10):5036-40.
  3. Shyti R, Kohler I, Schoenmaker B, Derks RJ, Ferrari MD, Tolner EA, Mayboroda OA, van den Maagdenberg AM. Plasma metabolic profiling after cortical spreading depression in a transgenic mouse model of hemiplegic migraine by capillary electrophoresis--mass spectrometry. Mol Biosyst. 2015 May;11(5):1462-71.

  4. Kloos D, Lingeman H, Mayboroda OA, Deelder AM, Niessen WM, Giera M. Chromatography-mass spectrometry based analysis of biologically active endogenous carboxylic acids. Trends Anal. Chem. 2014 Aug 61:17-28.

  5. Meissner A, van der Plas AA, van Dasselaar NT, Deelder AM, van Hilten JJ, Mayboroda OA. 1H-NMR metabolic profiling of cerebrospinal fluid in patients with complex regional pain syndrome-related dystonia. Pain. 2014 Jan;155(1):190-6.

  6. Klein-Wieringa IR, Andersen SN, Kwekkeboom JC, Giera M, de Lange-Brokaar BJ, van Osch GJ, Zuurmond AM, Stojanovic-Susulic V, Nelissen RG, Pijl H, Huizinga TW, Kloppenburg M, Toes RE, Ioan-Facsinay A. Adipocytes modulate the phenotype of human macrophages through secreted lipids. J Immunol. 2013 Aug 1;191(3):1356-63.

  7. Jónasdóttir HS, Nicolardi S, Jonker W, Derks R, Palmblad M, Ioan-Facsinay A, Toes R, van der Burgt YE, Deelder AM, Mayboroda OA, Giera M. Detection and structural elucidation of esterified oxylipids in human synovial fluid by electrospray ionization-fourier transform ion-cyclotron mass spectrometry and liquid chromatography-ion trap-MS(3): detection of esterified hydroxylated docosapentaenoic acid containing phospholipids. Anal Chem. 2013 Jun 18;85(12):6003-10.

  8. Kloos DP, Lingeman H, Niessen WM, Deelder AM, Giera M, Mayboroda OA. Evaluation of different column chemistries for fast urinary metabolic profiling. J Chromatogr B Analyt Technol Biomed Life Sci. 2013 May 15;927:90-6. 2013.

  9. Nevedomskaya E, Pacchiarotta T, Artemov A, Meissner A, van Nieuwkoop C, van Dissel JT, Mayboroda OA, Deelder AM. (1)H NMR-based metabolic profiling of urinary tract infection: combining multiple statistical models and clinical data. Metabolomics. 2012 Dec;8(6):1227-1235.