Metabolomics

Metabolomics is defined as the systemic analysis of the metabolome, which encompasses the totality of the metabolites in a biological system, i.e., the end-points and intermediate products of metabolism. Metabolomics has become a powerful diagnostic and prognostic tool to investigate, for example, susceptibility to disease, disease mechanisms and treatment outcome.

The metabolomics group houses several state-of-the art mass spectrometers ranging from triple-quadrupole to quadrupole time-of-flight mass analyzers. Our work focuses on the development and application of advanced analysis techniques for small molecules in the field of metabolomics. As main analytical techniques we use liquid chromatography (LC), including reversed-phase and hydrophilic interaction chromatography, and gas chromatography (GC) in combination with mass spectrometry as well as nuclear magnetic resonance spectroscopy (NMR) and advanced data analysis approaches. Next to global metabolic profiling (untargeted metabolomics), special emphasis is put on quantitative analysis using isotope-dilution approaches. Our state-of-the art analytical platforms are applied in collaboration with several groups (within and outside the LUMC) in a variety of research fields, such as inflammation, infection, obesity and migraine. Our main interests include lipid remodelling during acute inflammation, the effects of bioactive lipids on the course of inflammation, metabolic profiling in the field of biomarker discovery, metabolic changes during infection as well as the relation between altered metabolite profiles, inflammation, infection and disease.


Contact

Dr. Martin Giera
Phone: +31 715269527


Staff

Dr. Martin Giera
Dr. Oleg A. Mayboroda
Dr. Aswin Verhoeven
Dr. Rico Derks
Juan Alarcon Barrera, Msc.
Dr. Sarantos Kostidis
Ing. Evelyne Steenvoorden
Marieke Heijnk, Msc.


Key publications

Metabolite-Induced Protein Expression Guided by Metabolomics and Systems Biology.
Giera M, Branco dos Santos F, Siuzdak G
Cell Metabolism, 2018, 27, in press, available online February 2018

Butyrate reduces appetite and activates brown adipose tissue via the gut-brain neural circuit.
Li Z, Yi CX, Katiraei S, Kooijman S, Zhou E, Chung CK, Gao Y, van den Heuvel JK, Meijer OC, Berbée JFP, Heijink M, Giera M, Willems van Dijk K, Groen AK, Rensen PCN, Wang Y. Gut. 2017 Nov 3. pii: gutjnl-2017-314050. doi: 10.1136/gutjnl-2017-314050
 
Resolution of inflammation and sepsis survival are improved by dietary Ω-3 fatty acids.
Körner A, Schlegel M, Theurer J, Frohnmeyer H, Adolph M, Heijink M, Giera M, Rosenberger P, Mirakaj V. Cell Death Differ. 2018 Feb;25(2):421-431. doi: 10.1038/cdd.2017.177
 
Quantitative NMR analysis of intra- and extracellular metabolism of mammalian cells: A tutorial.
Kostidis S, Addie RD, Morreau H, Mayboroda OA, Giera M. Anal Chim Acta. 2017 Aug 8;980:1-24. doi: 10.1016/j.aca.2017.05.011
 
Automated quantification of metabolites in blood-derived samples by NMR.
Verhoeven A, Slagboom E, Wuhrer M, Giera M, Mayboroda OA. Anal Chim Acta. 2017 Jul 11;976:52-62. doi: 10.1016/j.aca.2017.04.013
 
Antifungal drug testing by combining minimal inhibitory concentration testing with target identification by gas chromatography-mass spectrometry.
Müller C, Binder U, Bracher F, Giera M. Nat Protoc. 2017 May;12(5):947-963. doi: 10.1038/nprot.2017.005
 
The hypoxanthine-xanthine oxidase axis is not involved in the initial phase of clinical transplantation-related ischemia-reperfusion injury.
Wijermars LG, Bakker JA, de Vries DK, van Noorden CJ, Bierau J, Kostidis S, Mayboroda OA, Tsikas D, Schaapherder AF, Lindeman JH. Am J Physiol Renal Physiol. 2017 Mar 1;312(3):F457-F464. doi: 10.1152/ajprenal.00214.2016
 
Analytical pitfalls and challenges in clinical metabolomics.
Kohler I, Verhoeven A, Derks RJ, Giera M. Bioanalysis. 2016 Jul;8(14):1509-32. doi: 10.4155/bio-2016-0090.
 
The neuroimmune guidance cue netrin-1 controls resolution programs and promotes liver regeneration.
Schlegel M, Köhler D, Körner A, Granja T, Straub A, Giera M, Mirakaj V. Hepatology. 2016 May;63(5):1689-705. doi: 10.1002/hep.28347
 
Jónasdóttir HS, Papan C, Fabritz S, Balas L, Durand T, Hardardottir I, Freysdottir J, Giera M.
Differential mobility separation of leukotrienes and protectins. Anal Chem. 2015 May 19;87(10):5036-40.