Dr. Guinevere S.M. Lageveen-Kammeijer

Researcher

Area(s) of interest


Introduction

Guinevere S.M. Lageveen-Kammeijer is a post-doctoral researcher at the Glycomics group at the Center for Proteomics. She defended my thesis: “Unravelling the Sugar-Coating of Prostate-Specific Antigen – Method Development and its Application to Prostate Cancer Research” in 2019 and established her own research line on identifying potential biomarkers of prostate cancer, where she particularly concentrates on changes in post-translational modifications on prostate-specific antigen and other tumor antigens.

Her research interest lie in the glycomics field and the technology capillary electrophoresis hyphenated with mass spectrometry. She is interested in the analysis of various biofluids for the identification of potential glycobiomarkers for several diseases (e.g. cancer and inflammation). Preferably using capillary electrophoresis hyphenated with mass spectrometry to gain in-depth knowledge at high sensitivity. 

 

 

 

 


Work Experience

2019-pres : Post-doctoral researcher, Leiden University Medical Center, Department Center for Proteomics and Metabolomics working on the development of a Prostate-Specific Antigen Glycomics Assay and its application to Prostate Cancer Research, Leiden, The Netherlands
2013-2019 : PhD candidate, Leiden University Medical Center, Department Center for Proteomics and Metabolomics, Leiden, The Netherlands which lead to the thesis entitled: “Unravelling the Sugar-Coating of Prostate-Specific Antigen – Method 
2018 : Visiting scientist, Northeastern University, Department Chemical and Biological Analysis, Boston, United States. Working on the development and evaluation of analytical approaches for the characterization of protein charge and proteoform heterogeneity using CE-ESI-MS under supervision of Prof. Dr. Alexander Ivanov
2017-pres : Member of the Organization Committee of The Netherlands Area Biotech (NLab) Discussion Group
2018-pres : Scientific committee member of the Glycomics session as well as the Early Career Scientist at Mass Spectrometry Applications to the Clinical Lab (MSACL) EU conference
2019-pres :  Member of the Werkgroep Scheidingsmethoden which is part of the Section Analytical Chemistry of the KNCV


Relevant Honours and Awards


CASSS Student Travel Grant, MicroScale Bioseparations (MSB), Pudong Shanghai, China2015 
China NVMS conference attendance fund, MicroScale Bioseparations (MSB), Pudong Shanghai, 2015 
(MSACL), EU, Salzburg, Austria Young Investigator Travel Grant, Mass Spectrometry Applications to the Clinical Lab 2015 
Center for Proteomics and Metabolomics at the Leiden University Medical Center. A collaboration between the Urology department of Amsterdam Medical Center and the  glycosylation profiling of prostate-specific antigen for early diagnosis of prostate cancer”.  Fellowship of Astellas Pharma B.V to support the following research: “High-resolution 2015 
(MSACL), Palm Springs, CA, USA oung Investigator Travel Grant, Mass Spectrometry Applications to the Clinical Lab  Y2017
Boston, MA, USA CASSS Student Travel Grant, Practical Applications of Mass Spectrometry (Mass Spec). 2017 
Center. Center and the Center for Proteomics and Metabolomics at the Leiden University Medical  prostate cancer”. A collaboration between the Urology department of Amsterdam Medical  “High-resolution glycosylation profiling of prostate-specific antigen for early diagnosis of Fellowship of the Cure for Cancer foundation to further support the following research: 2017 
(MSACL), EU, Salzburg, Austria Young Investigator Travel Grant, Mass Spectrometry Applications to the Clinical Lab 2018 

2014 CASSS Student Travel Grant, MicroScale Bioseparations (MSB), Pecs, Hungary


Publications

Books

Lageveen-Kammeijer, G.S.M.Unravelling the sugar-coating of prostate-specific antigen: Method development and its application to prostate cancer research. Thesis, 2019, ISBN: 978-94-6380-249-9.

International (refereed) journals

Peer-reviewed publications: 14 | H-index:  11 | i10-index: 12   | Citations: 521 |ORCID | Google Scholar

 Lageveen-Kammeijer, G.S.M.*, Haan, de N.*, Mohaupt, P., Wagt, A.P., Filius, M., Nouta, J., Falck, D., Wuhrer, M. Highly Sensitive CE-ESI-MS Analysis of N-Glycans from Complex Biological Samples . Nature Communications, 2019, 10, 2137. O.

Kammeijer, G.S.M., Nouta, J., Rosette, de la J.J.M.C.H, Reijke, de T.M., Wuhrer, M. An In-depth Glycosylation Assay for Urinary Prostate-Specific Antigen . Analytical Chemistry, 2018, 90 (7), pp 4414-4421, DOI: 10.1021/acs.analchem.7b04281. O.

Kammeijer, G.S.M., Nouta, J., Rosette, de la J.J.M.C.H, Reijke, de T.M., Wuhrer, M. An In-depth Glycosylation Assay for Urinary Prostate-Specific Antigen . Analytical Chemistry, 2018, 90 (7), pp 4414-4421, DOI: 10.1021/acs.analchem.7b04281. O.

Kammeijer, G.S.M., Jansen, B.C., Kohler, I., Heemskerk, A.A.M., Mayboroda, O.A., Hensbergen, P.J., Schappler, J., Wuhrer, M. Sialic Acid Linkage Differentiation of Glycopeptides using Capillary Electrophoresis – Electrospray Ionization – Mass Spectrometry . Scientific Reports, 2017, 7, 3733, DOI: 10.1038/s41598-017-03838-y. O.

Kammeijer, G.S.M., Kohler, I., Jansen, B.C., Hensbergen, P.J., Mayboroda, O.A., Falck, D. Wuhrer, M. Dopant Enriched Nitrogen Gas Combined with Sheathless Capillary Electrophoresis – Electrospray Ionization - Mass Spectrometry for Improved Sensitivity and Repeatability in Glycopeptide Analysis . Analytical Chemistry, 2016, 88 (11), pp 5849-5856, DOI: 10.1021/acs. analchem.6b00479. O.

Madunic, K., Zhang, T., Mayboroda, O.A., Holst, S., Stavenhagen, K., Jin, Chunsheng, Karlsson, N.G., Lageveen-Kammeijer, G.S.M., Wuhrer, M. Colorectal cancer cell lines show striking diversity of their O-glycome reflecting the cellular differentiation phenotype. Cellular and Molecular Life Sciences, 2020, pp 1-14, DOI:10.1007/s00018-020-03504-z. O.

Rebello, O.D., Nicolardi, Lageveen-Kammeijer, G.S.M., Nouta, J., Gardner, R.A., Mesker, W.E., Tollenaar, R.A.E.M., Spencer, D.I.R., Wuhrer, M., Falck, D. A Matrix-Assisted Laser Desorption/Ionization—Mass Spectrometry Assay for the Relative Quantitation of Antennary Fucosylated N-Glycans in Human Plasma. Frontiers in Chemistry, 2020, 8 (138), pp 1-14, DOI:10.3389/fchem.2020.00138. O.

Leoz, de M.L.A., … Haan, de N., Falck, D., Lageveen-Kammeijer, G.S.M., Wuhrer, M., … Stein, S.E. NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Analytical Methods. Molecular & Cellular Proteomics, 2020, 19 (1), pp 11-30, DOI: 10.1074/mcp.RA119.001677. O.

Sánchez López, E., Kammeijer, G.S.M., Crego, A.L., Luisa Marina, M., Ramautar, R., Peters, D.J.M., Mayboroda, O.A., Sheathless CE-MS Based Metabolic Profiling of Kidney Tissue Section Samples from a Mouse Model of Polycystic Kidney Disease. Scientific Reports, 2019 (9), 806, DOI: 10.1038/s41598- 018-37512-8. O.

Clerc, F., Novokmet, M., Dotz, V., Reiding, K.R., Haan, de N., Kammeijer, G.S.M., Dalebout, H., Bladergroen, M.R., Vukovic, F., Rapp, E. IBD-BIOM consortium, Targan, S.R., Barron, G., Manetti, N., Latiano, A., McGovern, D.P.B., Annese, V., Lauc, G., Wuhrer, M. Plasma N-Glycan Signatures of Inflammatory Bowel Diseases. Gastroenterology, 2018, 155 (3), pp 829-843, DOI: 10.1053/j. gastro.2018.05.030. O.

Šimurina, M., Haan, de N., … Fawkes, A., Kammeijer, G.S.M., Vojta, A., … Wuhrer, M., Lauc, G. Glycosylation of immunoglobulin G associates with clinical features of inflammatory bowel diseases, Gastroenterology, 2018, 154 (5), pp 1320-1333.e10, DOI: 10.1053/j.gastro.2018.01.002.

Clerc, F., Reiding, K.R., Jansen, B.C., Kammeijer, G.S.M., Bondt, A., Wuhrer, M. Human Plasma Protein N-Glycosylation. Glycoconjugate journal, 2016, 33 (3), pp 309-343, DOI: 10.1007/ s10719-015-9626-2. O.

Bondt, A., Nicolardi, S., Jansen, B.C., Stavenhagen, K., Blank, D., Kammeijer, G.S.M., Kozak, R.P., Fernandes, D.L., Hensbergen, P.J., Hazes, J.M.W., van der Burgt, Y.E.M., Dolhain, R.J.E.M., Wuhrer, M. Longitudinal Monitoring of Immunoglobulin A Glycosylation During Pregnancy by Simultaneous MALDI-FTICR-MS Analysis of N- and O-Glycopeptides. Scientific Reports, 2016 (6), 27955, DOI: 10.1038/srep23296. O.

Jansen, B.C., Bondt, A., Reiding, L., Lonardi, E., Jong, de C.J., Falck, D., Kammeijer, G.S.M., Dolhain, R.J.E.M., Rombouts, Y., Wuhrer, M. Pregnancy-Associated Serum N-Glycome Changes Studied by High-Throughput MALDI-TOF-MS.Scientific Reports, 2016 (6), 23296, DOI: 10.1038/srep23296. O.

Plomp, R., Dekkers, G., Rombouts, Y., Visser, R., Koeleman, C.A.M., Kammeijer, G.S.M., Jansen, B.C., Rispens, T., Hensbergen, P.J., Vidarsson, G., Wuhrer, M. Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3).Molecular and Cellular Proteomics, 2015. 14 (5): p. 1373- 1384, DOI: 10.1074/mcp.M114.047381. O.

* Authors contributed equally

** O: open access

Other; Technical Notes

Kammeijer, G.S.M., Kohler, I., Jansen, B.C., Hensbergen, P.J., Mayboroda, O.A., Falck, D., Lock, S., Wuhrer, M. Improved CESI-MS sensitivity and repeatability in Glycopeptide Analysis using a Dopant Enriched Nitrogen Gas. Document nr: RUO-MKT-02-4920-A, 2016 (Technical Note).

Kammeijer, G.S.M., Kohler, I., Jansen, B.C., Hensbergen, P.J., Mayboroda, O.A., Falck, D., Lock, S., Wuhrer, M. Separation and Detection of Glycopeptide Isomers with Differentially Linked Sialic Acids by CESI-MS. (Technical Note).  


Contact

Leiden University Medical Center
Building 1
Room P-01-065

Albinusdreef 2
2333 ZA Leiden
Tel: +31 71 526 6993

Postalzone P1-Q
P.O. Box 9600
2300 RC Leiden
The Netherlands

E-mail: g.s.m.kammeijer@lumc.nl