Dr. Guinevere S.M. Lageveen-Kammeijer

Senior Scientist

Area(s) of interest

  • Glycomics and Glycoproteomics
  • Analytical Chemistry
  • Mass Spectrometry
  • Assay Development
  • Biomarker Discovery

Introduction

Guinevere S.M. Lageveen-Kammeijer is a senior scientist at the Glyco(proteo)mics group at the Center for Proteomics. She defended her thesis: “Unravelling the Sugar-Coating of Prostate-Specific Antigen – Method Development and its Application to Prostate Cancer Research” in 2019 and established her own research line on identifying potential biomarkers of prostate cancer, where she particularly concentrates on changes in post-translational modifications on prostate-specific antigen and other tumor antigens.

Dr. Lageveen-Kammeijer is an internationally recognized expert in the characterization and identification of  potential glyco(proteo)mic biomarkers for several diseases (e.g. cancer and inflammation) in various biofluids with the final aim to translate her findings into the clinics. Moreover, she has extensive experience in improving glyco(proteo)mic methods for various analytical platforms which are mass spectrometry based. Particularly using capillary electrophoresis hyphenated with mass spectrometry to gain in-depth knowledge at high sensitivity. 


Work Experience

2020-Pres: Senior Scientist, Leiden University Medical Center, Department Center for Proteomics and Metabolomics working on the characterization and identification of  potential glyco(proteo)mic biomarkers for several cancers (e.g. prostate cancer and colorectal) in various biofluids with the final aim to translate her findings into the clinics.

2019-2020: Post-doctoral researcher, Leiden University Medical Center, Department Center for Proteomics and Metabolomics working on the development of a Prostate-Specific Antigen Glycomics Assay and its application to Prostate Cancer Research, Leiden, The Netherlands

2013-2019 : PhD candidate, Leiden University Medical Center, Department Center for Proteomics and Metabolomics, Leiden, The Netherlands which lead to the thesis entitled: “Unravelling the Sugar-Coating of Prostate-Specific Antigen – Method Development and its Application to Prostate Cancer Research”.

2018: Visiting scientist, Northeastern University, Department Chemical and Biological Analysis, Boston, United States. Working on the development and evaluation of analytical approaches for the characterization of protein charge and proteoform heterogeneity using CE-ESI-MS under supervision of Prof. Dr. Alexander Ivanov

2017-pres: Member of the Organization Committee of The Netherlands Area Biotech (NLab) Discussion Group

2018-pres: Scientific committee member of the Glycomics session as well as the Early Career Scientist at Mass Spectrometry Applications to the Clinical Lab (MSACL) EU conference

2019-pres:  Member of the Werkgroep Scheidingsmethoden which is part of the Section Analytical Chemistry of the KNCV

 


Relevant Honours and Awards

2020 Investigator Sponsored Research by Astellas. A three year project on the "Evaluation of Prostate-Specific Antigen Glycomics Assay for the Early Detection of Prostate Cancer

2018 Young Investigator Travel Grant, Mass Spectrometry Applications to the Clinical Lab (MSACL), EU, Salzburg, Austria

2017 Fellowship of the Cure for Cancer foundation to further support the following research: “High-resolution glycosylation profiling of prostate-specific antigen for early diagnosis of prostate cancer”. A collaboration between the Urology department of Amsterdam Medical Center and the Center for Proteomics and Metabolomics at the Leiden University Medical Center.

2017 CASSS Student Travel Grant, Practical Applications of Mass Spectrometry (Mass Spec). Boston, MA, USA

2017 Young Investigator Travel Grant, Mass Spectrometry Applications to the Clinical Lab (MSACL), Palm Springs, CA, USA

2015 Fellowship of Astellas Pharma B.V to support the following research: “High-resolution glycosylation profiling of prostate-specific antigen for early diagnosis of prostate cancer”. A collaboration between the Urology department of Amsterdam Medical Center and the Center for Proteomics and Metabolomics at the Leiden University Medical Center.

2015 Young Investigator Travel Grant, Mass Spectrometry Applications to the Clinical Lab (MSACL), EU, Salzburg, Austria

2015 NVMS conference attendance fund, MicroScale Bioseparations (MSB), Pudong Shanghai, China

2015 CASSS Student Travel Grant, MicroScale Bioseparations (MSB), Pudong Shanghai, China

2014 CASSS Student Travel Grant, MicroScale Bioseparations (MSB), Pecs, Hungary


Publications

Publications: 22 | H-index:  15 | Citations: 1091 | ORCID Google Scholar

INTERNATIONAL (REFEREED) JOURNALS

Last authorships 

  1.  
    1. Moran, A.B., Dominguez-Vega, E., Nouta, J., Pongracz, T., Reijke, T.M. de, Wuhrer, M., Lageveen-Kammeijer, G.S.M. (2021). Profiling the proteoforms of urinary prostate-specific antigen by capillary electrophoresis–mass spectrometry. J. of Proteomics. 238, 104148. DOI: 10.1016/j.prot.2021.104148. 
    2. Pont, L., Kuzyk, V., Benavente, F., Sanz-Nebot, V., Mayboroda, O.A., Wuhrer, M., Lageveen-Kammeijer, G.S.M. (2021). Site-Specific N-Linked Glycosylation Analysis of Human Carcinoembryonic Antigen by Sheathless Capillary Electrophoresis-Tandem Mass Spectrometry. J. Proteome Res. 2021, 20 (3), pp 1666-1675. DOI: 10.1021/acs.jproteome.0c00875.
    3. Madunic, K., Wagt, S., Zhang, T., Wuhrer, M., Lageveen-Kammeijer, G.S.M. Dopant-enriched nitrogen gas for enhanced electrospray ionization of released glycans in negative ion mode. Anal. Chem. 2021, 93, 18, 6919-6923. DOI: 10.1021/acs.analchem.1c00023
    4. Wang, W., Kaluza, A., Nouta, J., Nicolardi, S., Ferens-Sieczkowska, M., Wuhrer, M., Lageveen-Kammeijer, G.S.M.*, Haan, N. de*,  High-throughput glycopeptide profiling of prostate-specific antigen from seminal plasma by MALDI-MS. Talanta, 2021, 222, 121495, DOI: 10.1016/j.talanta.2020.121495.  

First authorships 

  1. Lageveen-Kammeijer, G.S.M., Kuster, B., Reusch, D., Wuhrer, M., High Sensitivity Glycomics in Biomedicine. Mass Spectrometry Reviews. 2021, E21730. DOI: 10.1002/mas.21730 
  2. Lageveen-Kammeijer, G.S.M.*, Haan, de N.*, Mohaupt, P., Wagt, A.P., Filius, M., Nouta, J., Falck, D., Wuhrer, M. Highly Sensitive CE-ESI-MS Analysis of N-Glycans from Complex Biological Samples. Nature Communications, 2019, 10, 2137
  3. Kammeijer, G.S.M., Nouta, J., Rosette, de la J.J.M.C.H, Reijke, de T.M., Wuhrer, M. An In-depth Glycosylation Assay for Urinary Prostate-Specific Antigen. Analytical Chemistry, 2018, 90 (7), pp 4414-4421, DOI: 10.1021/acs.analchem.7b04281. 
  4. Kammeijer, G.S.M., Jansen, B.C., Kohler, I., Heemskerk, A.A.M., Mayboroda, O.A., Hensbergen, P.J., Schappler, J., Wuhrer, M. Sialic Acid Linkage Differentiation of Glycopeptides using Capillary Electrophoresis – Electrospray Ionization – Mass Spectrometry. Scientific Reports, 2017, 7, 3733, DOI: 10.1038/s41598-017-03838-y. 
  5. Kammeijer, G.S.M., Kohler, I., Jansen, B.C., Hensbergen, P.J., Mayboroda, O.A., Falck, D. Wuhrer, M. Dopant Enriched Nitrogen Gas Combined with Sheathless Capillary Electrophoresis – Electrospray Ionization - Mass Spectrometry for Improved Sensitivity and Repeatability in Glycopeptide AnalysisAnalytical Chemistry, 2016, 88 (11), pp 5849-5856, DOI: 10.1021/acs. analchem.6b00479. 

 

Co-authorships

  1. Wang, D., Zhang, T, Madunic, K., de Waard, A.A., Blöchl, C., Mayboroda, O.A., Griffioen, M., Spaapen, R.M., Huber, C.G., Lageveen-Kammeijer, G.S.M., Wuhrer, M. (2022) Glycosphingolipid-Glycan Signatures of Acute Myeloid Leukemia Cell Lines Reflect Hematopoietic Differentiation. J. Proteome Res. 2022, Just accepted. DOI: 10.1021/acs.jproteome.1c00911.
  2. Blöchl, C., Wang, D., Madunic, K., Lageveen-Kammeijer, G.S.M., Huber, C.G., Wuhrer, M., Zhang. Integrated N- and O-Glycomics of Acute Myeloid Leukemia (AML) Cell Lines. Cells, 2021, 10 (1), 3058. DOI: 10.3390/cells10113058. 
  3. Leoz, de M.L.A., … Haan, de N., Falck, D., Lageveen-Kammeijer, G.S.M., Wuhrer, M., … Stein, S.E. NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Analytical Methods. Molecular & Cellular Proteomics, 2020, 19 (1), p. 11-30,  DOI: 10.1074/mcp.RA119.001677
  4. Rebello, O.D., Nicolardi, S., Lageveen-Kammeijer, G.S.M., Nouta, J., Gardner, R.A., Mesker, W.A., Tollenaar, R.A.E.M., Spencer, D.I.R., Wuhrer, M., Falck, D. A matrix-assisted laser desorption/ionization – mass spectrometry assay for the relative quantitation of antennary fucosylated N-glycans in human plasma, Frontiers in Chemistry, 2020, 138 (8), DOI: 10.3389/fchem.2020.00138.
  5. Drouin, N., … Lageveen-Kammeijer, G.S.M., Nouta, J., … Ramautar, R.  Capillary electrophoresis-Mass spectrometry at Trial by Metabo-ring: Effective electrophoretic mobility for Reproducible and Robust Compound Annotation. Analytical Chemistry, 2020, 92 (20), pp 14103-14112, DOI: 10.1021/acs.analchem.0c03129.
  6. Madunic, K., Zhang, T., Mayboroda, O.A., Holst, S., Stavenhagen, K., Jin, C., Karlsson, N., Lageveen-Kammeijer, G.S.M., Wuhrer, M. Colorectal cancer cell lines show striking diversity of their O-glycome. Cellular and Molecular Life Sciences, 2020, 78 (1), pp 337-350. DOI:10.1007/s00018-020-03504-z
  7. Sánchez López, E., Kammeijer, G.S.M., Crego, A.L., Luisa Marina, M., Ramautar, R., Peters, D.J.M., Mayboroda, O.A., Sheathless CE-MS Based Metabolic Profiling of Kidney Tissue Section Samples from a Mouse Model of Polycystic Kidney Disease. Scientific Reports, 2019 (9), 806, DOI: 10.1038/s41598- 018-37512-8. 
  8. Clerc, F., Novokmet, M., Dotz, V., Reiding, K.R., Haan, de N., Kammeijer, G.S.M., Dalebout, H., Bladergroen, M.R., Vukovic, 1F., Rapp, E. IBD-BIOM consortium, Targan, S.R., Barron, G., Manetti, N., Latiano, A., McGovern, D.P.B., Annese, V., Lauc, G., Wuhrer, M. Plasma N-Glycan Signatures of Inflammatory Bowel Diseases. Gastroentrology, 2018, 155 (3), pp 829-843, DOI: 10.1053/j. gastro.2018.05.030.
  9. Šimurina, M., ... Kammeijer, G.S.M., ... Wuhrer, M., Lauc, G. Glycosylation of immunoglobulin G associates with clinical features of inflammatory bowel diseases, Gastroenterology, 2018. 154 (5): p. 1320-1333.e10,  DOI: 10.1053/j.gastro.2018.01.002.
  10. Bondt, A., Nicolardi, S., Jansen, B.C., Stavenhagen, K., Blank, D., Kammeijer, G.S.M., Kozak, R.P., Fernandes, D.L., Hensbergen, P.J., Hazes, J.M.W., van der Burgt, Y.E.M., Dolhain, R.J.E.M., Wuhrer, M. Longitudinal Monitoring of Immunoglobulin A Glycosylation During Pregnancy by Simultaneous MALDI-FTICR-MS Analysis of N- and O-Glycopeptides. Scientific Reports, 2016 (6), 27955, DOI: 10.1038/srep23296. 
  11. Jansen, B.C., Bondt, A., Reiding, L., Lonardi, E., Jong, de C.J., Falck, D., Kammeijer, G.S.M., Dolhain, R.J.E.M.,              Rombouts, Y., Wuhrer, M. Pregnancy-Associated Serum N-Glycome Changes Studied by High-Throughput MALDI-TOF-MS. Scientific Reports, 2016 (6), 23296, DOI: 10.1038/srep23296. 
  12. Clerc, F., Reiding, K.R., Jansen, B.C., Kammeijer, G.S.M., Bondt, A., Wuhrer, M. Human Plasma Protein N-Glycosylation. Glycoconjugate journal, 2016, 33 (3), pp 309-343, DOI: 10.1007/ s10719-015-9626-2. 
  13. Plomp, R., Dekkers, G., Rombouts, Y., Visser, R., Koeleman, C.A.M., Kammeijer, G.S.M., Jansen, B.C., Rispens, T., Hensbergen, P.J., Vidarsson, G., Wuhrer, M. Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3). Molecular and Cellular Proteomics, 2015, 14 (5), pp. 1373- 1384, DOI: 10.1074/mcp.M114.047381. 

BOOKS 

  1. Lageveen-Kammeijer, G.S.M. Unravelling the sugar-coating of prostate-specific antigen: Method development and its application to prostate cancer research. Thesis, 2019, ISBN: 978-94-6380-249-9
  2. Wang, W., Wang, W. and Lageveen-Kammeijer, G.S.M. CE-MS approaches for glyco(proteo)mic analysis. CE-MS for Proteomics and Metabolomics, Wiley-Verlag. In Press
  3. Wang, W. and Lageveen-Kammeijer, G.S.M. High Sensitivity Glycoproteomic Analysis of Biological Samples by CE-ESI-MS. Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols, Methods in Molecular Biology, Springer Nature. In Press 
 OTHER 
  1. Kammeijer, G.S.M., Kohler, I., Jansen, B.C., Hensbergen, P.J., Mayboroda, O.A., Falck, D., Lock, S., Wuhrer, M. Improved CESI-MS sensitivity and repeatability in Glycopeptide Analysis using a Dopant Enriched Nitrogen Gas. Document nr: RUO-MKT-02-4920-A, 2016 (Technical Note). 
  2. Kammeijer, G.S.M., Kohler, I., Jansen, B.C., Hensbergen, P.J., Mayboroda, O.A., Falck, D., Lock, S., Wuhrer, M. Separation and Detection of Glycopeptide Isomers with Differentially Linked Sialic Acids by CESI-MS. (Technical Note). 


Contact

Leiden University Medical Center
Building 1
Room P-01-065

Albinusdreef 2
2333 ZA Leiden
Tel: +31 71 526 6993

Postalzone P1-Q
P.O. Box 9600
2300 RC Leiden
The Netherlands

E-mail: g.s.m.kammeijer@lumc.nl