Dr. Bram Heijs

Head Mass Spectrometry Imaging group


  • Mass Spectrometry Imaging

Curriculum Vitae


2017 - present

Groupleader of the Mass Spectrometry Imaging group at the Center for Proteomics & Metabolomics, Leiden, The Netherlands

2013 - present

PhD candidate at Leiden University Medical, Center for Proteomics & Metabolomics, Leiden, The Netherlands
PhD defense: 01-02-2018

Research project: MALDI mass spectrometry imaging on clinically challenging soft tissue sarcomas.

2013 - 2013 

Short ter scientific mission at Sheffield Hallam University, Biomedical Research Centre, Sheffield, United Kingdom

Research project: On-tissue digestion to reveal the peptide fingerprint of mouse brain & tumour tissue - 2 weeks

2012 - 2013 

MSc intern at Uppsala University, Medical Mass Spectrometry, Uppsala, Sweden

Research project: Mass Spectrometry-based analysis of the cerebrospinal fluid peptidome in search of biomarkers for Parkinson's Disease - 7 months

2011 - 2012

MSc intern at Leiden University, Leiden Institute of Chemistry, Leiden, The Netherlands

Research project: Purification of the PTD4-apoptin protein - 9 months

2008 - 2009 

BSc intern at Leiden University Medical Center, Department of Human Genetics, Leiden, The Netherlands

Research project: Microarray-based genomic selection for targeted DNA sequencing - 3 months



2010 - 2013

Master of Science, Life Science & Technology, Leiden University, Leiden, The Netherlands

2005 - 2011 

Bachelor of Science, Life Science & Technology, Leiden University, Leiden, The Netherlands/Technical University Delft, Delft, The Netherlands



Bram Heijs is groupleader of the Mass Spectrometry Imaging group at the Center for Proteomics and Metabolomics. Research interests are developing novel methodologies for clinical MSI, and the application of MSI to solve biochemical/biomedical research questions.


Ràfols, P., Vilalta, D., Torres, S., Calavia, R., Heijs, B., McDonnell, L., Brezmes, J.,  del Castillo, E., Yanes, O., Ramírez, N., Correig, X. (2018) Assessing the potential of sputtered gold nanolayers in mass spectrometry imaging for metabolomics applications. PLoS One. In Press.

Dilillo, M., Heijs, B., McDonnell, L.A. (2018) Mass spectrometry imaging: How will it affect clinical research in the future? Expert Reviews of Proteomics 15(9): 709-716. doi:10.1080/14789450.2018.1521278.

Buck, A., Heijs, B., Beine, B., Schepers, J., Cassese, A., Heeren, R., McDonnell, L., Henkel, C., Walch, A., Balluff, B. (2018) Round Robin Study of Formalin-Fixed Paraffin-Embedded Tissues in Mass Spectrometry Imaging, Analytical and Bioanalytical Chemistry, doi: 10.1007/s00216-018-1216-2.

Piga, I., Heijs, B., Nicolardi, S., Giusti, L., Marselli, L., Marchetti, P., Mazzoni, M.R., Lucacchini, A., McDonnell, L.A. (2017) Ultra-high resolution MALDI-FTICR-MSI analysis of intact proteins in mouse and human pancreas tissue. International Journal of Mass Spectrometry, in press. doi:10.1016/j.ijms.2017.11.001.

Heijs, B., Holst, S., Briaire-de Bruijn, I.H., van Pelt, G.W., de Ru, A.H., van Veelen, P.A., Mesker, W.E., Tollenaar, R.A., Drake, R.R., Mehta, A.S., Bovée, J.V.M.G., Wuhrer, M., McDonnell, L.A. (2016) Multimodal mass spectrometry imaging of N-glycans and proteins from the same tissue section. Analytical Chemistry 88(15): 7745-7753. doi:10.1021/acs.analchem.6b01739.

Holst, S., Heijs, B., de Haan, N., van Zeijl, R.J.M., Briaire-de Bruijn, I.H., van Pelt, G.W., Metha, A.S., Mesker, W.E., Tollenaar, R.A., Drake, R.R., Bovée, J.V.M.G., McDonnell, L.A., Wuhrer, M. (2016). Linkage-specific in-situ sialic acid derivatization for N-glycan mass spectrometry imaging of formalin-fixed paraffin-embedded tissues. Analytical Chemistry 88(11): 5904-5913. doi:10.1021/acs.analchem.6b00819

Heijs, B., Abdelmoula, W.M., Lou, S., Briaire-de Bruijn, I.H., Dijkstra, J., Bovée, J.V.M.G., McDonnell, L.A. (2015). Histology-guided high resolution MALDI mass spectrometry imaging. Analytical Chemistry 87(24): 11978-11983. doi:10.1021/acs.analchem.5b03610.

Heijs, B., Tolner, E.A., Bovée, J.V.M.G., van den Maagdenberg, A.M.J.M., McDonnell, L.A. (2015). Brain region-specific dynamics of on-tissue protein digestion using MALDI Mass Spectrometry Imaging. Journal of Proteome Research 14(12): 5348-5354 doi:10.1021/acs.jproteome.5b00849.

Heijs, B., Carreira, R.J., Tolner, E.A., de Ru, A.H., van den Maagdenberg, A.M.J.M., van Veelen, P.A., McDonnell. L.A. (2015). Comprehensive characterization of the mouse brain proteome sampled in mass spectrometry imaging studies. Analytical Chemistry 87(3): 1867-1875. doi:10.1021/ac503952q.





Leids Universitair Medisch Centrum
Gebouw 1
Kamer P-01-067

Albinusdreef 2
2333 ZA Leiden
Tel: +31 71 5264154

Postzone P1-Q
Postbus 9600
2300 RC Leiden

E-mail: b.p.a.m.heijs@lumc.nl