Vaccine development

Principal Investigator(s)

Dr. Marjolein Kikkert, Prof. Dr. Eric J. Snijder

Research topics

  • DUB-knockout viruses as nidovirus vaccines
  • Yellow fever virus 17D-based vaccine vectors

Key publications

  • Ad26 vector-based COVID-19 vaccine encoding a prefusion-stabilized SARS-CoV-2 Spike immunogen induces potent humoral and cellular immune responses.  Rinke Bos, Lucy Rutten, Joan E. M. van der Lubbe, Mark J. G. Bakkers, Gijs Hardenberg, Frank Wegmann, David Zuijdgeest, Adriaan H. de Wilde, Annemart Koornneef, Annemiek Verwilligen, Danielle van Manen, Ted Kwaks, Ronald Vogels, Tim J. Dalebout, Sebenzile K. Myeni, Marjolein Kikkert, Eric J. Snijder, Zhenfeng Li, Dan H. Barouch, Jort Vellinga, Johannes P. M. Langedijk, Roland C. Zahn, Jerome Custers & Hanneke Schuitemaker. npj Vaccines  5,: 91 (2020) https://doi.org/10.1038/s41541-020-00243-x
  • SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology.  Ogando NS, Dalebout TJ, Zevenhoven-Dobbe JC, Limpens RWAL, van der Meer Y, Caly L, Druce J, de Vries JJC, Kikkert M, Bárcena M, Sidorov I, Snijder EJ. J Gen Virol. 2020 Jun 22
  • Mechanism and structural diversity of exoribonuclease-resistant RNA structures in flaviviral RNAs. MacFadden A, O'Donoghue Z, Silva PAGC, Chapman EG, Olsthoorn RC, Sterken MG, Pijlman GP, Bredenbeek PJ, Kieft JS. Nat Commun. 2018 Jan 9;9(1):119
  • Crystal structure of the Middle East respiratory syndrome coronavirus (MERS-CoV) papain-like protease bound to ubiquitin facilitates targeted disruption of deubiquitinating activity to demonstrate its role in innate immune suppression. Bailey-Elkin BA, Knaap RC, Johnson GG, Dalebout TJ, Ninaber DK, van Kasteren PB, Bredenbeek PJ, Snijder EJ, Kikkert M, Mark BL. J Biol Chem. 2014; 289:34667-82
  • Molecular and immunological characterization of a DNA-launched yellow fever virus 17D infectious clone. Jiang X, Dalebout TJ, Lukashevich IS, Bredenbeek PJ, Franco D. J Gen Virol. 2015 Apr;96(Pt 4):804-14
  • Deubiquitinase function of arterivirus papain-like protease 2 suppresses the innate immune response in infected host cells. van Kasteren PB, Bailey-Elkin BA, James TW, Ninaber DK, Beugeling C, Khajehpour M, Snijder EJ, Mark BL, Kikkert M. Proc Natl Acad Sci USA. 2013; 110:E838-47
  • Yellow fever 17D-vectored vaccines expressing Lassa virus GP1 and GP2 glycoproteins provide protection against fatal disease in guinea pigs. Jiang X, Dalebout TJ, Bredenbeek PJ, Carrion R Jr, Brasky K, Patterson J, Goicochea M, Bryant J, Salvato MS, Lukashevich IS. Vaccine. 2011 Feb 1;29(6):1248-57.