Dr. P.H. Nibbering

Associate Professor

Vakgebied(en)


Onderzoek

Mission: to develop strategies (and agents) to 1) prevent and treat infections with anti-infective resistant pathogens and 2) promote healing of chronic (infected) wounds.

Focus: Pathogen host interactions, stimulation of the innate immune system to enhance its defence against infections, biofilm formation, intracellular bacteria, new generations antimicrobial peptides, herbal medicines and medicinal larvae. Both translational and basic research.

Techniques: Assays in microbiology (various antibacterial assays, biofilms on abiotic and biotic surfaces, bead diffusion assays, antifungal and antiviral activities), immunology (macrophage differentiation, monocyte and granulocyte functional activities, macrophage-T cell co-cultures), and cell biology (3-D organotypic cultures, medicinal larvae).


Publicaties

van der Plas MJ, van Dissel JT, Nibbering PH. Maggot secretions skew monocyte-macrophage differentiation away from a pro-inflammatory to a pro-angiogenic type. PLoS One. 2009 Nov 30;4(11):e8071. doi:10.1371/journal.pone.0008071.

van der Plas MJ, Baldry M, van Dissel JT, Jukema GN, Nibbering PH. Maggot secretions suppress pro-inflammatory responses of human monocytes through elevation of cyclic AMP. Diabetologia. 2009; 52:1962-1970. doi: 10.1007/s00125-009-1432-6.

van der Does AM, Beekhuizen H, Ravensbergen B, Vos T, Ottenhoff TH, van Dissel JT, Drijfhout JW, Hiemstra PS, Nibbering PH. LL-37 directs macrophage differentiation toward macrophages with a proinflammatory signature. J Immunol. 2010; 185:1442-1449. doi: 10.4049/jimmunol.1000376.

van der Does AM, Bogaards SJ, Ravensbergen B, Beekhuizen H, van Dissel JT, Nibbering PH. Antimicrobial peptide hLF1-11 directs granulocyte-macrophage colony-stimulating factor-driven monocyte differentiation toward macrophages with enhanced recognition and clearance of pathogens. Antimicrob Agents Chemother. 2010;54:811-816. doi: 10.1128/AAC.00652-09.

van der Does AM, Joosten SA, Vroomans E, Bogaards SJ, van Meijgaarden KE, Ottenhoff TH, van Dissel JT, Nibbering PH. The antimicrobial peptide hLF1-11 drives monocyte-dendritic cell differentiation toward dendritic cells that promote antifungal responses and enhance Th17 polarization. J Innate Immun. 2012; 4:284-292. doi: 10.1159/000332941.

van der Does AM, Hensbergen PJ, Bogaards SJ, Cansoy M, Deelder AM, van Leeuwen HC, Drijfhout JW, van Dissel JT, Nibbering PH. The human lactoferrin-derived peptide hLF1-11 exerts immunomodulatory effects by specific inhibition of myeloperoxidase activity. J Immunol. 2012;188: 5012-5019. doi: 10.4049/jimmunol.1102777.

Peleg AY, de Breij A, Adams MD, Cerqueira GM, Mocali S, Galardini M, Nibbering PH, Earl AM, Ward DV, Paterson DL, Seifert H, Dijkshoorn L. The success of acinetobacter species; genetic, metabolic and virulence attributes. PLoS One. 2012;7(10):e46984. doi: 10.1371/journal.pone.0046984.

de Breij A, Eveillard M, Dijkshoorn L, van den Broek PJ, Nibbering PH, Joly-Guillou ML. Differences in Acinetobacter baumannii strains and host innate immune response determine morbidity and mortality in experimental pneumonia. PLoS One. 2012;7(2):e30673. doi: 10.1371/journal.pone.0030673.

Koning RI, de Breij A, Oostergetel GT, Nibbering PH, Koster AJ, Dijkshoorn L. Cryo-electron tomography analysis of membrane vesicles from Acinetobacter baumannii ATCC19606 T. Res Microbiol. 2013;164:397-405. doi: 10.1016/j.resmic.2013.02.007.

Klik hier voor een overview van alle publicaties.


Biosketch

Following my masters in Biology (B4) at the University of Utrecht, I did my PhD in immunology at  the University of Leiden (Quantitative immunocytochemical characterization of murine mononuclear phagocytes). Thereafter, I continued to work at the Dept. of Infectious Diseases of the Leiden University Medical Center (LUMC) and meanwhile obtained certification in Immunology and in Medical Microbiology from  the SMBWO. Currently I am a senior researcher and lecturer at the LUMC.

My current research focusses on 1) the development of new agents/strategies for the treatment of infections with multidrug-resistant pathogens, 2) pathogen host interactions, and 3) identification and mode of action of bioactive components in medicinal larval secretions.

 

Current grants are:

1.Topical treatment with a cathelicidin-based AMP as a novel approach to eradicate MRSA nasal carriage: ZonMW (95110078).

2.Biofilm alliance (Bali): EU; FP7-Health 2011.

3.  Innovative antimicrobial peptides to improve the treatment of burn wounds. TKI-LSH-Impuls 2014.

4. Synthetic biology and genomics platform for new-tonature bioactive peptides (SYNGENOPEP) Fund New Chemical Innovation NCI-TA 2014.001.

5.  developmen of a novel nasal therapy containing antimicrobial peptides to treat MRSA nasal carriage (NAS-AMP). Eurostars-2. 2015. 

 


Contactgegevens

Leids Universitair Medisch Centrum
Gebouw 1
Kamer C5-40

Albinusdreef 2
2333 ZA Leiden
Tel: +31 71 5262204

Postzone C5-P
Postbus 9600
2300 RC Leiden

E-mail: P.H.Nibbering@LUMC.nl