Dr. P.H. Nibbering

Associate Professor

Onderzoek

Mission: to develop strategies (and agents) to 1) prevent and treat bacterial/fungal infections not treatable with current anti-infectives and 2) promote healing of chronic (infected) wounds.

Focus: Pathogen host interactions, stimulation of the innate immune system to enhance its defense against infections, biofilm formation, intracellular bacteria, persisters, design and testing of synthetic antimicrobial peptides, and medicinal larvae. Both translational and basic research.

Techniques: Microbiology (various antibacterial and antifungal assays, biofilms on abiotic and biotic surfaces, models for persisters), immunology (macrophage differentiation, monocyte and granulocyte functional activities, macrophage-T cell co-cultures), and cell biology (isolation and culture of cells, 3-D organotypic cultures, medicinal larvae).



Publicaties

 

de Breij A, Riool M, Cordfunke RA, Malanovic N, de Boer L, Koning RI, Ravensbergen E, Franken M, van der Heijde T, Boekema BK, Kwakman PHS, Kamp N, El Ghalbzouri A, Lohner K, Zaat SAJ, Drijfhout JW, Nibbering PH. The antimicrobial peptide SAAP-148 combats drug-resistant bacteria and biofilms.


Sci Transl Med. 2018 Jan 10;10(423). pii: eaan4044. doi: 10.1126/scitranslmed.aan4044


Riool M, de Breij A, de Boer L, Kwakman PHS, Cordfunke RA, Cohen O, Malanovic N, Emanuel N, Lohner K, Drijfhout JW, Nibbering PH, Zaat SAJ. Controlled Release of LL-37-Derived Synthetic Antimicrobial and Anti-Biofilm Peptides SAAP-145 and SAAP-276 Prevents Experimental Biomaterial-Associated Staphylococcus aureus Infection. Adv Funct Mat 27, 16066223, 2017.


 


Riool M, de Breij A, Drijfhout JW, Nibbering PH, Zaat SAJ. Antimicrobial Peptides in Biomedical Device Manufacturing. Front Chem. 2017 Aug 24;5:63. doi: 10.3389/fchem.2017.00063. eCollection 2017. Review.


 


 


Excretions/secretions from medicinal larvae (Lucilia sericata) inhibit complement activation by two mechanisms.Tamura T, Cazander G, Rooijakkers SH, Trouw LA, Nibbering PH. Wound Repair Regen. 2017 Jan;25(1):41-50. doi: 10.1111/wrr.12504.


 


Antimicrobial Peptide P60.4Ac-Containing Creams and Gel for Eradication of Methicillin-Resistant Staphylococcus aureus from Cultured Skin and Airway Epithelial Surfaces. Haisma EM, Göblyös A, Ravensbergen B, Adriaans AE, Cordfunke RA, Schrumpf J, Limpens RW, Schimmel KJ, den Hartigh J, Hiemstra PS, Drijfhout JW, El Ghalbzouri A, Nibbering PH. Antimicrob Agents Chemother. 2016 Jun 20;60(7):4063-72. doi: 10.1128/AAC.03001-15.


 


Correction: Detection of Alpha-Toxin and Other Virulence Factors in Biofilms of Staphylococcus aureus on Polystyrene and a Human Epidermal Model.


den Reijer PM, Haisma EM, Lemmens-den Toom NA, Willemse J, Koning RI, Demmers JA, Dekkers DH, Rijkers E, El Ghalbzouri A, Nibbering PH, van Wamel W.


PLoS One. 2016 Mar 24;11(3):e0152544. doi: 10.1371/journal.pone.0152544.


 


The licorice pentacyclic triterpenoid component 18β-glycyrrhetinic acid enhances the activity of antibiotics against strains of methicillin-resistant Staphylococcus aureus.


de Breij A, Karnaoukh TG, Schrumpf J, Hiemstra PS, Nibbering PH, van Dissel JT, de Visser PC. Eur J Clin Microbiol Infect Dis. 2016 Apr;35(4):555-62. doi: 10.1007/s10096-015-2570-z.


 


Detection of Alpha-Toxin and Other Virulence Factors in Biofilms of Staphylococcus aureus on Polystyrene and a Human Epidermal Model. den Reijer PM, Haisma EM, Lemmens-den Toom NA, Willemse J, Koning RI, Demmers JA, Dekkers DH, Rijkers E, El Ghalbzouri A, Nibbering PH, van Wamel W. PLoS One. 2016 Jan 7;11(1):e0145722. doi: 10.1371/journal.pone.0145722.


 


Prevention of Staphylococcus aureus biomaterial-associated infections using a polymer-lipid coating containing the antimicrobial peptide OP-145.


de Breij A, Riool M, Kwakman PH, de Boer L, Cordfunke RA, Drijfhout JW, Cohen O, Emanuel N, Zaat SA, Nibbering PH, Moriarty TF. J Control Release. 2016 Jan 28;222:1-8. doi: 10.1016/j.jconrel.2015.12.003.

        

Klik hier voor een overview van alle publicaties.


Biosketch

Following my masters in Biology (B4) at the University of Utrecht, I did my PhD in immunology at  the University of Leiden (Quantitative immunocytochemical characterization of murine mononuclear phagocytes). Thereafter, I continued to work at the Dept. of Infectious Diseases of the Leiden University Medical Center (LUMC) and meanwhile obtained certification in Immunology and in Medical Microbiology from  the SMBWO. Currently I am a senior researcher and lecturer at the LUMC.

My current research focusses on 1) the development of new agents/strategies for the treatment of infections with multidrug-resistant pathogens, 2) pathogen host interactions, and 3) identification and mode of action of bioactive components in medicinal larval secretions.

 

Current grants are:

  

1. Development of a novel nasal therapy containing antimicrobial peptides to treat MRSA nasal carriage (NAS-AMP). Eurostars-2. 2015. 


2. Synthetic biology and genomics platform for new-tonature bioactive peptides (SYNGENOPEP) Fund New Chemical Innovation NCI-TA 2014.001. 2016.


3 .   Next stage development of the novel synthetic antimicrobial peptide SAAP-148 (NESDAP). NWO domain Applied and Engineering Sciences (TTW), Research programme NACTAR, dossier 16434. 2018.


4. Wound infections: Formulation of Innovative Antimicrobial Peptide preparations (WIFI-APP). TKI-LSH. 2018.

 

 


Contactgegevens

Leids Universitair Medisch Centrum
Gebouw 1
Kamer C5-40

Albinusdreef 2
2333 ZA Leiden
Tel: +31 71 5262204

Postzone C5-P
Postbus 9600
2300 RC Leiden

E-mail: P.H.Nibbering@LUMC.nl