Targeted proteomics for coagulation assessment

Proteins belonging to the coagulation and fibrinolytic system are essential to hemostasis, warranting prevention of blood loss and contributing to repair of the injured vessel wall. The critical role of the individual proteins in normal hemostasis typically becomes evident in individuals with deficiencies, as it may result in either a bleeding phenotype or predisposition to thrombosis. Moreover, coagulation- and fibrinolysis-related proteins interplay with a number of other important pathways (

Multiplexed Targeted Proteomics to Quantitatively Assess Plasma Coagulation Proteins in Individuals Patients and Cohorts Studies

Well-established laboratory tests are available to assess the presence and functionality of various (anti-)coagulation and fibrinolysis proteins. However, these tests do not allow high multiplicity, require large volume of plasma and are often costly. A novel technology to quantify plasma proteins is mass spectrometry-based quantitative targeted proteomics (MS-QTP). Through stable isotope-labeled internal standards it is possible to absolutely quantify a large number of proteins in one single analytical run, requiring less than 50 µL of plasma.

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Themes for innovation

Team Members

  • Bart J.M. van Vlijmen, PhD, Associate professor
  • Ing. Richard J. Dirven, Technician

The MS-QTP work is part of interdepartmental LUMC-project and is hosted by the Department of Clinical Chemistry and Laboratory Medicine (head Prof.dr. Christa Cobbaert). This interdepartemental LUMC-project involves:

The department of Clinical Chemistry and Laboratory Medicine ( Prof.dr. Christa Cobbaert and Dr. Renee Ruhaak)

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Team Members

  • Bart J.M. van Vlijmen, PhD, Associate professor
  • Ing. Richard J. Dirven, Technician

The MS-QTP work is part of interdepartmental LUMC-project and is hosted by the Department of Clinical Chemistry and Laboratory Medicine (head Prof.dr. Christa Cobbaert). This interdepartemental LUMC-project involves:

The department of Clinical Chemistry and Laboratory Medicine ( Prof.dr. Christa Cobbaert and Dr. Renee Ruhaak)

The department of Internal Medicine (Prof.dr, Ton Rabelink, Prof.dr Henri Versteeg, Prof.dr. Jeroen Eikenboom, Prof.dr Erik Klok)

The department of Clinical Epidemiology (Prof.dr Suzanne Cannegieter, Prof.dr Frits Rosendaal)

The Center for Proteomics and Metabolomics (Prof.dr. Manfred Wuhrer and Dr. Yassene Mohammed)

Key publications