Dr. Paul J. Hensbergen


  • Proteomics


Main interests:

  • Microbial proteomics

  • Proteases

  • Cancer glycoproteomics


Paul Hensbergen obtained his PhD in animal ecotoxicology in 1999 at the Vrije University in Amsterdam (promotor prof. dr. N.M. van Straalen). During his PhD, he studied metal binding proteins in the springtail Orchesella cincta in relation to heavy metal tolerance. Then, he joined the Department of Dermatology as a postdoc, first at the Vrije University Medical Center (VUMC), later at the Leiden University Medical Center (LUMC), where he studied the potential of cytotoxic T-cell attracting chemokines as anti-tumor molecules (Dutch Cancer Fund project). He also purified and characterized endogenous chemokines secreted by human skin cells and investigated their proteolytic processing and activity. Later, he was appointed as senior scientist in the same department where he implemented proteomics technologies to study skin diseases. In 2004, he moved to the Center for Proteomics and Metabolomics (CPM) of the LUMC where he is now an associate professor. His work is focused on using mass spectrometry-based proteomics to understand cellular processes involved in disease. His main focus is on microbial proteomics, proteases, and, more recently, cancer glycoproteomics (Marie Curie European Training Network GlyCoCan, https://glycocan.eu/). He also coordinates the CPM teaching activities for Biomedical Science students in Leiden and he is a member of the Executive Body of the Master Biomedical Sciences. Moreover, he is a member of the steering committee of the Netherlands Proteomics Platform.

Five key publications of last 2 years

Hensbergen, P. J. ; Klychnikov, O. I., Bakker, D., Dragan, I., Kelly, M.L., Minton, N.P., Corver, J., Kuijper, E. J., Drijfhout, J. W., van Leeuwen, H. C. Clostridium difficile secreted Pro-Pro endopeptidase PPEP-1 (ZMP1/CD2830) modulates adhesion through cleavage of the collagen binding protein CD2831. FEBS Letters 2015, 589, 3952-3958

Fleurbaaij, F.; Heemskerk, A. A.; Russcher, A.; Klychnikov, O. I.; Deelder, A. M.; Mayboroda, O. A.; Kuijper, E. J.; van Leeuwen, H. C.; Hensbergen, P. J. Capillary-electrophoresis mass spectrometry for the detection of carbapenemases in (multi-)drug-resistant Gram-negative bacteria. Anal. Chem. 2014, 86, 9154-9161.

van Leeuwen, H. C.; Klychnikov, O. I.; Menks, M. A.; Kuijper, E. J.; Drijfhout, J. W.; Hensbergen, P. J. Clostridium difficile sortase recognizes a (S/P)PXTG sequence motif and can accommodate diaminopimelic acid as a substrate for transpeptidation. FEBS Lett. 2014, 588 , 4325-4333.

Plomp, R.; Hensbergen, P. J.; Rombouts, Y.; Zauner, G.; Dragan, I.; Koeleman, C. A.; Deelder, A. M.; Wuhrer, M. Site-specific N-glycosylation analysis of human immunoglobulin e. J. Proteome. Res. 2014, 13, 536-546.

Hensbergen, P. J. ; Klychnikov, O. I.; Bakker, D.; van Winden, V. J.; Ras, N.; Kemp, A. C.; Cordfunke, R. A.; Dragan, I.; Deelder, A. M.; Kuijper, E. J.; Corver, J.; Drijfhout, J. W.; van Leeuwen, H. C. A novel secreted metalloprotease (CD2830) from Clostridium difficile cleaves specific proline sequences in LPXTG cell surface proteins. Mol. Cell Proteomics. 2014, 13, 1231-1244.

All publications:


Leids Universitair Medisch Centrum
Gebouw 1
Kamer L-04-074

Albinusdreef 2
2333 ZA Leiden
Tel: +31(0)715266394

Postzone S3
Postbus 9600
2300 RC Leiden

E-mail: p.j.hensbergen@lumc.nl