Dr. Bruno Guigas


  • Assistant Professor
  • Leiden Parasite Immunology Group (PARA)
  • Aging and Signal transduction (MCB)


I started my scientific training at the University of Grenoble (France) with a Master’s degree in Exercise Physiology and Bioenergetics, followed by a PhD internship in the group of Pr. dr. X. Leverve (LBFA, University of Grenoble, France) where I studied the mechanisms of action of the antidiabetic drug metformin. After graduating, I joined one of the European leading group in the field of AMPK signalling and intermediary metabolism at the de Duve Institute (Pr. dr. L. Hue, Brussels, Belgium). Supported by a Michel de Visscher Research Fellowship, I studied the role of AMPK in the control of hepatic glucose metabolism and mitochondrial oxidative phosphorylation. In 2007, I moved to the Netherlands and joined the department of Molecular Cell Biology of the Leiden University Medical Center for driving several research projects aiming to identify novel therapeutic strategies for metabolic disorders. From 2010, I am one of the PI of the Diabetes group, in the MCB section ‘Aging and Signal Transduction’ of Prof. dr. Peter ten Dijke. Since 2012, I’m also appointed as Assistant Professor at the Leiden Parasite Immunology Group, headed by Prof. dr. Maria Yazdanbakhsh, where I’m currently developing a new research line at the interface between immunology and metabolism .

Key publications:

  • Foretz M, Guigas B, Bertrand L, Pollak M, Viollet B. Metformin: from mechanism of action to therapy. (2014). Cell Metabolism , 20(6):953-966 .
  • Wijngaarden MA*, Bakker LEH*, van der Zon GC, Jazet I, t’Hoen PB, Willems van Dijk K, Pijl H, Guigas B. Regulation of skeletal muscle energy/nutrient-sensing pathways during metabolic adaptation to fasting in healthy humans. (2014). AJP-Endocrinology & Metabolism, 307(10):E885-95.
  • Geerling JJ*, Boon MR*, van der Zon GC, van den Berg SAA, van den Hoek AM, Lombes M, Princen HM, Havekes LM, Rensen PCN*, Guigas B*. Metformin reduces plasma cholesterol and triglyceride levels by promoting VLDL-triglyceride clearance by brown adipose tissue in mice. (2014). Diabetes, 63(3):880:91.
  • Wijngaarden MA, van der Zon GC, Willems van Dijk K, Pijl H, Guigas B. Effects of prolonged fasting on AMP-activated protein kinase signaling, metabolic gene expression and mitochondrial content in skeletal muscle from lean and obese individuals. (2013). AJP-Endocrinology & Metabolism , 304(9):E1012-21.
  • Stephenne X, Foretz M, Taleux N, van der Zon GC, Sokal EM, Hue L, Viollet B, Guigas B. Metformin activates AMP-activated protein kinase in primary human hepatocytes by decreasing cellular energy status. (2011). Diabetologia , 54(12):3101-10.
  • Linssen MML, Van Raalte DH, Toonen EJM, Alkema W, Van Der Zon GC, Dokter WH, Diamant M, Ouwens DM*, Guigas B*. Prednisolone-induced beta cell dysfunction is associated with impaired endoplasmic reticulum homeostasis in INS-1E cells. (2011). Cellular Signaling, 23(11):1708-15.
  • De Leeuw Van Weenen JE, Parlevliet ET, Maechler P, Romijn JA, Ouwens DM, Pijl H*, Guigas B*. The dopamine receptor D2 agonist bromocriptine inhibits glucose-stimulated insulin secretion mainly by direct activation of the α2-adrenergic receptors in beta cells. (2010). Biochemical Pharmacology, 79(12):1827-36.
  • Viollet B, Guigas B, Leclerc J, Hebrard S, Lantier L, Mounier R, Andreelli F, Foretz M. AMPK in the regulation of hepatic energy metabolism: from physiology to therapeutic perspectives. (2009). Acta Physiologica , 196(1):81-98. Review
  • Taleux N, De Potter I, Deransart C, Lacraz G, Favier R, Leverve X, Hue L, Guigas B. Lack of starvation-induced activation of AMP-activated protein kinase in the hypothalamus of the Lou/C rats resistant to obesity. (2008). International Journal of Obesity , 32(4):639-47.
  • Guigas B, Taleux N, Foretz M, Detaille D, Andreelli F, Viollet B, Hue L. AMP-activated protein kinase-independent inhibition of hepatic mitochondrial oxidative phosphorylation by AICA riboside. (2007). Biochemical Journal , 404(3):499-507.
  • Stephenne X, Najimi M, Ngoc DK, Smets F, Hue L, Sokal EM*, Guigas B*. Cryopreservation of human hepatocytes alters the mitochondrial respiratory chain complex I. (2007). Cell Transplantation , 16(4):409-419.
  • Guigas B, Naboulsi R, Taleux N, Villanueva G, Lopez-Novoa JM, Leverve XM, El-Mir MY. The flavonoïd silibinin decreases hepatic gluconeogenesis by an inhibitory effect on glucose-6-phosphatase. (2007). Cellular Physiology and Biochemistry , 20(6):925-34.
  • Guigas B, Bertrand L, Taleux N, Foretz M, Wiernsperger N, Vertommen D, Andreelli F, Viollet B, Hue L. 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside and metformin inhibit hepatic glucose phosphorylation by an AMP-activated protein kinase-independent effect on glucokinase translocation. (2006). Diabetes , 55:865-874.
  • Detaille D, Guigas B, Chauvin C, Batandier C, Fontaine E, Wiernsperger N, Leverve XM. Metformin prevents high glucose-induced human endothelial cell death through a mitochondrial permeability transition-dependent process. (2005). Diabetes, 54(7):2179-87.
  • Guigas B, Detaille D, Chauvin C, Batandier C, De Oliveira F, Fontaine E, Leverve XM. Metformin inhibits permeability transition and cellular death: a pharmacological in vitro study. (2004). Biochemical Journal, 382(3):877-884.

* contributed equally 


Leids Universitair Medisch Centrum
Gebouw 1
Kamer P-04-25

Albinusdreef 2
2333 ZA Leiden
Tel: 071 5261328

Postzone L4-Q
Postbus 9600
2300 RC Leiden

E-mail: b.g.a.guigas@lumc.nl