Dr. Yuri E.M. van der Burgt

Vakgebied(en)

  • Glycomics and glycoproteomics
  • FTICR mass spectrometry
  • Clinical chemistry proteomics
  • Cancer diagnosis and monitoring
  • Analytical chemistry
  • Assay development

Introductie

 

 

Yuri van der Burgt obtained his Ph.D. in bio-organic chemistry at Utrecht University in the Netherlands in 1999. At the Leiden University Medical Center (LUMC) he started his work in the new field of MS-based proteomics and contributed to the establishment of a glycoproteomics facility. After his postdoc he worked for three years as a scientist at Crucell, where he was responsible for the analysis of recombinant proteins. To strengthen his expertise in the field of biomolecular MS he moved to the FOM-Institute in Amsterdam to work on biomarker discovery within the Netherlands Proteomics Center (NPC). Then, to follow his ambitions in clinical research he returned to the LUMC as an associate professor at the Center for Proteomics and Metabolomics. He is responsible for multiple MS-platforms that are applied both for in-depth analyses and high-throughput clinical proteomics studies. In collaboration with the department of Clinical Chemistry he is involved in transfer of quantitative clinical chemistry proteomics (qccp) methods into routine assays.

 

 


Publications (Method Development)

Automation of High-Throughput Mass Spectrometry-Based Plasma N-Glycome Analysis with Linkage-Specific Sialic Acid Esterification.Bladergroen MR, Reiding KR, Hipgrave Ederveen AL, Vreeker GC, Clerc F, Holst S, Bondt A, Wuhrer M, van der Burgt YE. J Proteome Res. 2015

 

Developments in FTICR-MS and Its Potential for Body Fluid Signatures.Nicolardi S, Bogdanov B, Deelder AM, Palmblad M, van der Burgt YE. Int J Mol Sci . 2015

 

Solid-phase extraction strategies to surmount body fluid sample complexity in high-throughput mass spectrometry-based proteomics.Bladergroen MR, van der Burgt YE. J Anal Methods Chem. 2015

 

In-Depth Characterization of Protein Disulfide Bonds by Online Liquid Chromatography-Electrochemistry-Mass Spectrometry.Switzar L, Nicolardi S, Rutten JW, Oberstein SA, Aartsma-Rus A, van der Burgt YE. J Am Soc Mass Spectrom. 2016  

 

Top-down MALDI-in-source decay-FTICR mass spectrometry of isotopically resolved proteins.

Nicolardi S, Switzar L, Deelder AM, Palmblad M, van der Burgt YE. Anal Chem. 2015

 

Structural analysis of an intact monoclonal antibody by online electrochemical reduction of disulfide bonds and Fourier transform ion cyclotron resonance mass spectrometry.Nicolardi S, Deelder AM, Palmblad M, van der Burgt YE. Anal Chem . 2014

 

On-line electrochemical reduction of disulfide bonds: improved FTICR-CID and -ETD coverage of oxytocin and hepcidin.Nicolardi S, Giera M, Kooijman P, Kraj A, Chervet JP, Deelder AM, van der Burgt YE. J Am Soc Mass Spectrom . 2013

 

Identification of new apolipoprotein-CIII glycoforms with ultrahigh resolution MALDI-FTICR mass spectrometry of human sera.Nicolardi S, van der Burgt YE, Dragan I, Hensbergen PJ, Deelder AM. J Proteome Res. 2013  

 

Mapping O-glycosylation of apolipoprotein C-III in MALDI-FT-ICR protein profiles.Nicolardi S, van der Burgt YE, Wuhrer M, Deelder AM. Proteomics. 2013

 

Standardized and automated solid-phase extraction procedures for high-throughput proteomics of body fluids. Bladergroen MR, Derks RJ, Nicolardi S, de Visser B, van Berloo S, van der Burgt YE, Deelder AM. J Proteomics. 2012  

 

Identification of human serum peptides in fourier transform ion cyclotron resonance precision profiles. Nicolardi S, Dalebout H, Bladergroen MR, Mesker WE, Tollenaar RA, Deelder AM, van der Burgt YE. Int J Proteomics. 2012  

 

Precision profiling and identification of human serum peptides using Fourier transform ion cyclotron resonance mass spectrometry.Nicolardi S, Palmblad M, Hensbergen PJ, Tollenaar RA, Deelder AM, van der Burgt YE. Rapid Commun Mass Spectrom . 2011

 

Quality control based on isotopic distributions for high-throughput MALDI-TOF and MALDI-FTICR serum peptide profiling.Nicolardi S, Palmblad M, Dalebout H, Bladergroen M, Tollenaar RA, Deelder AM, van der Burgt YE. J Am Soc Mass Spectrom . 2010


Publications (Clinical Proteomics)

Nicolardi, S.; Bladergroen, M.R.; Deelder, A.M.; Tollenaar, R.A.E.M.; Palmblad, M.; Mesker, W.E.; van der Burgt, Y.E.M. SPE-MALDI profiling of serum peptides and proteins by ultrahigh resolution FTICR-MS. Chromatographia 2015

 

Serum peptide signatures for pancreatic cancer based on mass spectrometry: a comparison to CA19-9 levels and routine imaging techniques. Velstra B, Vonk MA, Bonsing BA, Mertens BJ, Nicolardi S, Huijbers A, Vasen H, Deelder AM, Mesker WE, van der Burgt YE, Tollenaar RA. J Cancer Res Clin Oncol. 2015

 

Case-controlled identification of colorectal cancer based on proteomic profiles and the potential for screening. Huijbers A, Mesker WE, Mertens BJ, Bladergroen MR, Deelder AM, van der Burgt YE, Tollenaar RA. Colorectal Dis. 2014

 

Nicolardi, S.; Velstra, B.; Mertens, B.J.; Bonsing, B.; Mesker, W.E.; Tollenaar, R.A.E.M.; Deelder, A.M.; van der Burgt, Y.E.M. Ultrahigh resolution profiles lead to more detailed serum peptidome signatures of pancreatic cancer. Transl. Proteom. 2014

 

Detection of pancreatic cancer using serum protein profiling. Velstra B, Bonsing BA, Mertens BJ, van der Burgt YE, Huijbers A, Vasen H, Mesker WE, Deelder AM, Tollenaar RA. HPB (Oxford). 2013 

 

Improved classification of breast cancer peptide and protein profiles by combining two serum workup procedures. Velstra B, van der Burgt YE, Mertens BJ, Mesker WE, Deelder AM, Tollenaar RA. J Cancer Res Clin Oncol. 2012

 

Proteomic serum biomarkers and their potential application in cancer screening programs. Huijbers A, Velstra B, Dekker TJ, Mesker WE, van der Burgt YE, Mertens BJ, Deelder AM, Tollenaar RA. Int J Mol Sci. 2010


Publications (qCCP)

Automated Multiplex LC-MS/MS Assay for Quantifying Serum Apolipoproteins A-I, B, C-I, C-II, C-III, and E with Qualitative Apolipoprotein E Phenotyping.van den Broek I, Romijn FP, Nouta J, van der Laarse A, Drijfhout JW, Smit NP, van der Burgt YE, Cobbaert CM. Clin Chem. 2016

 

Quantification of serum apolipoproteins A-I and B-100 in clinical samples using an automated SISCAPA-MALDI-TOF-MS workflow.van den Broek I, Nouta J, Razavi M, Yip R, Bladergroen MR, Romijn FP, Smit NP, Drews O, Paape R, Suckau D, Deelder AM, van der Burgt YE, Pearson TW, Anderson NL, Cobbaert CM. Methods. 2015

 

Quantifying protein measurands by peptide measurements: where do errors arise?van den Broek I, Romijn FP, Smit NP, van der Laarse A, Drijfhout JW, van der Burgt YE, Cobbaert CM. J Proteome Res . 2015

 

Metrological traceability in mass spectrometry-based targeted protein quantitation: a proof-of-principle study for serum apolipoproteins A-I and B100.Smit NP, Romijn FP, van den Broek I, Drijfhout JW, Haex M, van der Laarse A, van der Burgt YE, Cobbaert CM. J Proteomics. 2014

 

Quality requirements for quantitative clinical chemistry proteomics. Smit N, van den Broek I, Romijn FP, Haex M, Deelder AM, van der Burgt Y, van der Laarse A, Cobbaert CM Transl Proteomics 2014

 

Evaluation of interspecimen trypsin digestion efficiency prior to multiple reaction monitoring-based absolute protein quantification with native protein calibrators.van den Broek I, Smit NP, Romijn FP, van der Laarse A, Deelder AM, van der Burgt YE, Cobbaert CM. J Proteome Res. 2013


Contactgegevens

Leids Universitair Medisch Centrum
Gebouw 2
Kamer R-06-024

Einthovenweg 20
2333 ZC Leiden
Tel: +31(0)715269527

Postzone S3
Postbus 9600
2300 RC Leiden

E-mail: y.e.m.van_der_burgt@lumc.nl