Osteoarchaeologist dr. Rachel Schats investigates traces of Malaria infection on human archaeologic skeletons under an NWO VENI grant.
At the Center for Proteomics and Metabolomics we assist her with the extraction and detection of hemozoin, a detergent of the malaria parasite, using mass spectrometry. After developing a robust method, samples of human remaining's from Middle Age Netherlands will be analysed, to create a geographic overview of Malaria infection during the Middle Age, a period in our history of which less is known concerning this topic.
January 23, 2020:
NWO LIFT project awarded: Proteoform-resolved pharmacokinetics of biopharmaceuticals
Proteoform-resolved pharmacokinetics of biopharmaceuticals
Lead applicant: Professor Manfred Wuhrer, LUMC
Consortium: LUMC, Roche Diagnostics GmbH
Modern medicines are often proteins administered into the bloodstream. These proteins will often undergo changes after this administration; for instance, they may lose part of their molecular structure, or this may become modified. The LUMC, working together with two international companies (Roche and Sciex), will devise methods to map the changes undergone by administered proteins, and then examine the influence these changes have, not just on the lifetime of the drug but also on its activity. The project will contribute towards quality control, improved drug applicability, and the development of new medicines.
October 3, 2019:
NWO KLEIN-1 project awarded: Cleaving like a Pro: PPEP (Pro-Pro endopeptidase)-regulated processes in Clostridiodes difficile
Cleaving like a Pro: PPEP (Pro-Pro endopeptidase)-regulated processes in Clostridiodes difficile
Dr P.J. Hensbergen (Leiden University Medical Center)
Clostridioides difficile, a bacterium that causes intestinal infections, secretes an enzyme (PPEP-1) that regulates the spread of the bacterium. In doing so it cleaves surface proteins from the bacteria, proteins which are necessary for attachment. However, in the case of one of these surface proteins, we have yet to discover what it attaches to. PPEPs also occur in other bacteria, but their activity is slightly different. C. difficile also has a second PPEP, but its function is unknown. In this project we want to further unpack the PPEP-regulated processes in C. difficile, and to better understand the activity of different PPEPs.