Services
Peptide synthesis
At the facility, peptides are routinely synthesised in the range of 5-80 amino acids, in an amount of 1-200 µmol (smaller and larger amounts are also possible). All are analysed by LC-MS and if needed, purified by RP-HPLC. For analytical purposes, the Peptide Core Facility has a Waters liquid chromatography electro spray mass spectrometer at its disposal. We routinely use Fmoc-solid-phase chemistry and have 2 Syro II robot synthesizers as well as an Intavis synthesizer equipped with real-time Fmoc-monitoring.
…At the facility, peptides are routinely synthesised in the range of 5-80 amino acids, in an amount of 1-200 µmol (smaller and larger amounts are also possible). All are analysed by LC-MS and if needed, purified by RP-HPLC. For analytical purposes, the Peptide Core Facility has a Waters liquid chromatography electro spray mass spectrometer at its disposal. We routinely use Fmoc-solid-phase chemistry and have 2 Syro II robot synthesizers as well as an Intavis synthesizer equipped with real-time Fmoc-monitoring.
One of the Syro's 48 peptides can be synthesized simultaneously on a typical 25 µmol scale. The other Syro can synthesize 6 X 96 peptides in up to 4 µmol amounts in one run for screening purposes. The Intavis synthesizer is used for synthesizing larger amounts of peptides and for fine-tuning difficult sequences using the Fmoc-monitoring methodology.
Besides 'straight forward' peptides, it’s also possible to synthesize specifically modified peptides:
- Ubiquitin(like) and derivatives thereof
- Variations at the N-terminus; biotinylation (with or without a spacer), acetylation, fluorophores, phosphorylated peptides
- Peptides with a side chain modification (such as thiolysine)
- UV cleavable peptides
- Unnatural amino acid incorporation
- Alanine scans