New 3D culture platform supports B-cell development for gene therapy research
The study, "ABO: A 3D stroma-supported culture platform enabling full human B-lymphopoiesis for disease modeling and gene therapy development," describes the Assay for B-cell Output (ABO), a 3D culture system that supports the development of human B cells from blood stem and progenitor cells through all major developmental stages. The platform also reproduces the developmental defects seen in cells from patients with RAG1-deficient severe combined immunodeficiency (SCID) and demonstrates correction following lentiviral gene therapy.
The ABO platform provides a valuable model for studying inherited immune disorders and for evaluating new gene therapy approaches before they reach the clinic. By improving preclinical disease modelling, the work supports Cure4Life's goal of making safe and effective gene therapies available to more patients.