Services

Are you interested in single-cell or single-molecule sequencing, whole genome sequencing or de novo assembly? Explore next generation sequencing possibilities at LGTC. We are happy to offer you the services listed below. As the possibilities are endless, what you are specifically looking for may not be described here. Please contact us to explore your options or request a quote via info@lgtc.nl.

Equipment

The LGTC has equipment that can be used for many different applications and is available for general or shared use. We offer several platforms as well as custom protocols for single-cell RNA, DNA, and multi-omic library preps. In addition, we have nearly 10 years of experience with long-read sequencing, and we offer the latest sequencing platforms from PacBio and Oxford Nanopore.

Sanger sequencing

We offer Sanger sequencing service with reads of approx. 900 base pairs. The samples are processed on an Applied Biosystems 96-capillary (ABI3730xl) system.

Training and education

Leiden Genome Technology Center (LGTC) staff organize several courses and lectures every year for members of the Medical Genetics Centre (MGC). These courses include:

Single cell data analysis

This week-long course covers the practicalities of single-cell sample preparation and analysis, with a particular focus on single-cell RNA-sequencing (scRNA-seq) libraries. This course is aimed at both wet-lab researchers interested in learning how to analyse their own single-cell data sets, as well as bioinformaticians who are new to single-cell sequencing analysis. Basic knowledge of the programming language R is a prerequisite for participating in the course.

Technology Facilities

This one-day course is directed at PhD students who just started their research, but other participants are also welcome. The program consists of short lectures explaining the different genomics technologies available at LUMC’s core facilities and Erasmus MC.

Next-generation sequencing data analysis

The aim of this course is to give a broad overview of varying NGS applications, focusing on different ways to analyse the data. Presentations will discuss the different choices available for data analysis, why a specific approach was selected, and which alternatives are available. Besides discussing and demonstrating some widely applicable tools, the course doesn’t include practical data analysis nor will specific algorithms be discussed in detail. For practical experience, participants should follow more specific courses (e.g. a course on RNAseq, de novo assembly, metagenomics, etc.) after this general introductory course.

Custom services

Have you not found what you are looking for? We can advise you on experimental design and offer support for custom protocol development. Please contact info@lgtc.nl.

Pricing

Request a quote via info@lgtc.nl.

Terms and conditions

Download the LGTC Terms and conditions as a PDF.