In September 2019, the LGTC acquired the PacBio Sequel II platform. The Sequel II is a single-molecule sequencer capable of sequencing long DNA fragments (up to 200kb read length).
Technology Sequencing on the PacBio Sequel II is based on Single Molecular Real Time (SMRT) technology. An active DNA polymerase is immobilized on the bottom of each of the 8 million wells (ZMW = zero-mode wavelength). Each time a nucleotide is incorporated, it’s held in place by the polymerase and a fluorescent signal is detected in real-time. The incorporated nucleotides are identified by the four different fluorescent labels corresponding to A, T, G, and C. The collection of data consists of recording a movie of light pulses (pulse data) which is then translated into bases (base data). More information about the technology can be found on pacb.com. You will receive CCS data in .bam file format.
De novo assembly of small and large genomes with 99.9999% accuracy
Survey large population cohort studies for structural variants
Sequence full-length transcriptomes or targeted transcripts
Target regions not currently accessible by other technologies
Detect genomic variation in complex populations on all size scales
Detect epigenetic modification
Oxford Nanopore MinION and Flongle
These sequencing platforms can be used for real-time DNA and RNA sequencing. The nanopore technology uses flow cells with nanopores - tiny holes embedded in an electro-resistant membrane. Each nanopore corresponds to its own electrode connected to a channel and sensor chip, which measures the electric current that flows through the nanopore. When a molecule passes through a nanopore, the current is disrupted to produce a characteristic ‘squiggle’. The squiggle is then decoded using base calling algorithms to determine the DNA or RNA sequence in real-time. More information about the technology can be found on nanoporetech.com.
The Oxford Nanopore PromethION We will provide PromethION services beginning January 2023. Please contact us for further information.