PacBio Sequel II
In September 2019, the LGTC acquired the PacBio Sequel II platform. The Sequel II is a single-molecule sequencer capable of sequencing long DNA fragments (up to 200kb read length).
Technology
Sequencing on the PacBio Sequel II is based on Single Molecular Real Time (SMRT) technology. An active DNA polymerase is immobilized on the bottom of each of the 8 million wells (ZMW = zero-mode wavelength). Each time a nucleotide is incorporated, it’s held in place by the polymerase and a fluorescent signal is detected in real-time. The incorporated nucleotides are identified by the four different fluorescent labels corresponding to A, T, G, and C. The collection of data consists of recording a movie of light pulses (pulse data) which is then translated into bases (base data). More information about the technology can be found on pacb.com.
You will receive CCS data in .bam file format.
Applications
- De novo assembly of small and large genomes with 99.9999% accuracy
- Survey large population cohort studies for structural variants
- Sequence full-length transcriptomes or targeted transcripts
- Target regions not currently accessible by other technologies
- Detect genomic variation in complex populations on all size scales
- Detect epigenetic modification
