The LUMC human iPSC Hotel: Generation of research grade human iPSCs and beyond

Primary cells for reprogramming

The LUMC hiPSC Hotel offers the isolation of primary cells from the following human tissue sources:

  • skin (punch biopsy, 4 mm): punch biopsies can be stored for up to two weeks in PBS at 4°C. Skin fibroblasts will be isolated from the dermis. Reprogramming with LV, episomal plasmids, SeV, RNA. Note that in the Netherlands punch biopsies are not permitted for minors.
  • Peripheral blood: storage for up to one day at RT. 
    • Peripheral blood mononuclear cells (PBMCs) are isolated from 10 ml (heparine tubes) and frozen (pause step). For reprogramming, erythroblasts are expanded from the PBMC fraction and are reprogrammed with LV or episomal plasmids, as requested.
    • Blood outgrowth endothelial cells (BOECs): a minimum of 80 ml peripheral blood is required. Under Dutch ethical approval not applicable for children (max. blood volume for collection is 40 ml). In general the success rate for isolation and the reprogramming efficiencies are low relative to other cell sources.  Reprogramming with LV.
  • Urine (~150 ml): immediate isolation of renal epithelial cells. Reprogramming with LV, episomal plasmids or SeV upon request. 
  • Milk teeth: immediate isolation of dental pulp cells. Reprogramming with LV.  

For the following cell types we offer reprogramming of ready-to-use cells:

  • lymphoblast cells (EBV-transformed); reprogramming with episomal vectors
  • chondrocytes; reprogramming with LV
  • ligament cells; reprogramming with LV
  • mesenchymal stromal (or stem) cells (from bone marrow); reprogramming with LV

Reprogramming