The LUMC human iPSC Hotel: Generation of research grade human iPSCs and beyond


Biomat on microfluidic chip

The Biomat consortium, consisting of different research groups in Flanders (Belgium) and the Netherlands, focuses on the development of innovative platforms for large scale testing of biomaterials in a 3D microenvironment (organ-on-a-chip). Vascularized bone, and skeletal and heart muscle from a variety of reporter hiPSC lines will be generated on microfluidic chips to enable testing of different biomaterials. 

Within the first two years of Biomat-on-Microfluidic Chip project, important steps towards creating a new generation of patient-specific organ-on-chip platforms were taken by developing several engineering and biological technologies.

  • First designs and models of the chip have been made in the form of inserts for culture plates. We have succeeded in forming three-dimensional biological micro-constructs of patient-specific cells and various biomaterials at micron scale. In addition to that, we have developed multiple robust bioassays for screening the toxicity/biocompatibility and the functionality of newly developed biomaterials.
  • Although this 3D model has been tested with human cell lines and stem cells, we have also optimized protocols for differentiating human-induced pluripotent stem cells (hiPSCs) towards muscle and bone tissues and for coupling these cells with reporters with which their growth and metabolism can be monitored non-invasively. Furthermore, we have shown the formation of 3D micro-tissues using these cells.
  • The development of the platform for vascularization of 3D micro-tissues has also been demonstrated.
  • In addition to that, we have developed a sensor that can be coupled to the chip in order to monitor tissue formation overtime.
  • We have developed a technology for fabrication of micro-biomaterials with different chemistries that can be screened in the platform for biocompatibility and biological functionality.

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