The LUMC hiPSC Hotel offers the generation of research grade human induced pluripotent stem cells (hiPSCs) from different tissue sources using state of the art reprogramming techniques. The service includes isolation of somatic cells, reprogramming into hiPSCs and basic characterization of pluripotency. We now also offer generation of isogenic control hiPSCs using CRISPR/Cas9 technology. Furthermore the Hotel supports hiPSC-based project planning and assay development, provides validated differentiation protocols and control hiPSCs, and organizes training courses on an annual basis. Our standard services are free of charge for LUMC researchers. Costs apply for external parties.
Service at a glance
- Isolation and banking of primary cells; mycoplasma testing
- Generation of hiPSCs using either integrating or non-integrating reprogramming vectors
- Expansion of ≥3 hiPSC clones* on Vitronectin XF and in TESR-E8 medium (Stem Cell Technologies); banking at passages ~ 4 and 6. *derived from separate hiPSC colonies; clonal origin has not been confirmed
- Characterization of 3 hiPSC clones: FACS analysis of pluripotency markers (SSEA4, OCT3/4, Nanog) in undifferentiated hiPSCs; IF staining of mesodermal, ectodermal and endodermal markers after short-term in vitro differentiation.
- Cell line authentication using Short Tandem Repeat (STR) analysis (LUMC dept. of Human Genetics)
- Additional characterization (e.g. karyotyping, PluriTest, ScoreCard) is available upon request (costs apply).
- Generation of isogenic hiPSCs using CRISPR/Cas9 technology
- Provision of ‘control’ hiPSCs (from individuals without known genetic disease; various options with respect to gender, age, reprogramming vector).
- Supply of differentiation protocols developed in house.
- Advice and support regarding hiPSC project planning, grant writing, assay development, collaborations etc.
- Training courses
What you need to start
Human tissue/ cells with appropriate informed consent which covers hiPSC research and commercial use if applicable (see lab resources).
Ethical approval from the LUMC CME.
Project description: 1-2 A4 with general patient information (anonymized), mutation, disease phenotype, number of samples (for internal use of the hiPSC Hotel).
License reprogramming vectors (for commercial use only).