Sequel Pacific Biosciences

Single Molecule, Real-Time DNA Sequencing System

In September 2016, the LGTC acquired the PacBio Sequel platform. The Sequel is a single molecule sequencer for the sequencing of long DNA fragments (up to 60kb read length).

Technology

Sequencing on the PacBio Sequel is based on Single Molecular Real Time (SMRT) technology. An active DNA polymerase is immobilized on the bottom of each of the 1 million wells (ZMW = zero mode wavelength). Each time a nucleotide is incorporated, it is held in place by the polymerase and a fluorescent signal is detected in real time. The incorporated nucleotides are identified by the four differnet fluorescent labels corresponding to A, T, G, and C. The collection of data consists of the recording of a movie of light pulses (pulse dta) which is then translated into bases (base data). More information about the technology can be found on the Pacific Biosciences website.

Output

You will receive .bam files.

Applications

  • De novo assembly of small and large genomes with 99.9999% accuracy
  • Survey large population cohort studies for structural variants
  • Sequence full-length transcriptomes or targeted transcripts
  • Target regions not currently accessible by other technologies
  • Detect genomic variation in complex populations on all size scales
  • Detect epigenetic modifications