LUMC Flow cytometry Core Facility (FCF)

Instruments

FCF instruments

The FCF houses all advanced LUMC’s research and diagnostic flow cytometry and mass cytometry instruments, currently containing over 24 instruments.

The FCF currently operates four standard conventional flow cytometers (up to 8 fluorochromes) and seven advanced conventional flow cytometers (up to 12 fluorochromes), including research-grade instruments located in biosafety level II and III laboratories (ML-II and ML-III) for analyzing biohazardous materials. In addition, the FCF contains five high-speed spectral flow cytometers, which can simultaneously measure up to either 25 (three instruments) or 40 fluorochromes (two instruments) per cell at speeds of 25,000 to 35,000 cells per second.

The FCF is also holding five cell sorters (up to 17 colors; two in Tender process), which can be used to sort specific subpopulations for further study and analysis.

In addition, the FCF has three mass cytometers (cytometry by time-of-flight, or CyTOF), which can simultaneously measure more than 50 “labels” (i.e., metals of different mass) per cell; two CyTOF instruments can also be converted to measure cells in situ in tissue sections.


Research ML-I & Diagnostic
Research ML-I & Diagnostic
Research ML-I
Research ML-I/III
Research ML-I/II & Diagnostic
Research ML-I/II 
Research