Equipment

We currently serve more than 500 users per year and houses over 24 instruments, making the FCF one of the largest flow cytometry core facilities in Europe and one of the top 10 facilities in the world.

Equipment overview

The LUMC FCF is equipped with over 24 advanced research and diagnostic flow cytometry instruments, including five cell sorters, three mass cytometers, and CE-IVD-certified Canto and Lyric systems for diagnostics and clinical trials. The facility also features specialized flow cytometers capable of analyzing biohazardous samples within BSL-II and BSL-III laboratories, providing cutting-edge technology to support a wide range of research and clinical needs.

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Below is an overview of our equipment.

	Instruments	Number of Instruments	Number of Lasers	Number of Parameters	Special Features	Biosafety Level
Conventional	BD Canto-II	4	3	8	96 well plate reader, CE-IVD	ML-I, ML-II
	BD LSRFortessa (X20)	2	4-5	16	NIR laser	ML-I
	BD Lyric	3	3	12	CE-IVD	Unclassified, BSL-3
Spectral	Cytek Aurora	5	3/5	25/>40	96/384 well plate reader, ESP	ML-I
	Sony D7000	2	5	>40	96/384 well plate reader, ESP	ML-I
	Beckman Coulter CytoFLEX LX + mosaic88	1	6	>40	96 well plate reader, IR laser	ML-I
Cell Sorters	BD FACSAria III	1	4	15		ML-I
	BD FACS Discover S8	1	5	>40	Imaging	ML-I
	Cytek Aurora CS	1	5	>40	ESP	ML-I
	Beckman Coulter CytoFLEX SRT	2	4	15		ML-I, ML-II
CyTOF	Standard Biotools CyTOF XT	1	-	50		Unclassified
CyTOF	Standard Biotools Hyperion	2	-	50	Spatial information	Unclassified

NIR = near-infrared, ESP = enhanced small particle detection, IR = infrared

Conventional analyzers

BD Canto-II

4 instruments
3 lasers
8 parameters
96 well plate reader
CE-IVD feature
Biosafety level ML-I, ML-II

BD LSRFortessa (X20)

2 instruments
4-5 lasers
16 parameters
Near-infrared laser
Biosafety level ML-I

BD Lyric

3 instruments
3 lasers
12 parameters
CE-IVD feature
Biosafety level unclassified, BSL-3

Spectral analyzers

Cytek Aurora

5 instruments
3/5 lasers
25/>40 parameters
96/384 well plate reader
ESP feature
Biosafety level ML-I

Sony ID7000

2 instruments
5 lasers
>40 parameters
96/384 well plate reader
Biosafety level ML-I

The Beckman Coulter CytoFLEX LX + mosaic88

Beckman Coulter CytoFLEX LX + mosaic88

1 instrument
6 lasers
>40 parameters
96 well plate reader
Infrared laser
Biosafety level ML-I

Cell sorters

BD FACSAria III

1 instrument
4 lasers
15 parameters
Biosafety level ML-I

BD FACS Discover S8

1 instrument
5 lasers
>40 parameters
Imaging feature
Biosafety level ML-I

Cytek Aurora CS

1 instrument
5 lasers
>40 parameters
Enhanced small particle detection feature
Biosafety level ML-I

Beckman Coulter CytoFLEX SRT

2 instruments
4 lasers
15 parameters
Biosafety level ML-I, ML-II

CyTOF

Standard Biotools CyTOF XT

1 instrument
50 parameters
Biosafety level unclassified

Standard Biotools Hyperion

2 instruments
50 parameters
Spatial information feature
Biosafety level unclassified

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