Publications

Core publications in glycobiology

Tegument Glycoproteins and Cathepsins of Newly Excysted Juvenile Fasciola hepatica Carry Mannosidic and Paucimannosidic N-glycans.
Garcia-Campos A, Ravidà A, Nguyen DL, Cwiklinski K, Dalton JP, Hokke CH, O'Neill S, Mulcahy G.
PLoS Negl Trop Dis. 2016 May 3;10(5):e0004688. doi: 10.1371/journal.pntd.0004688. eCollection 2016 May.

Fasciola hepatica Surface Coat Glycoproteins Contain Mannosylated and Phosphorylated N-glycans and Exhibit Immune Modulatory Properties Independent of the Mannose Receptor.
Ravidà A, Aldridge AM, Driessen NN, Heus FA, Hokke CH, O'Neill SM.
PLoS Negl Trop Dis. 2016 Apr 22;10(4):e0004601. doi: 10.1371/journal.pntd.0004601. eCollection 2016 Apr.

Community Rates of IgG4 Antibodies to Ascaris Haemoglobin Reflect Changes in Community Egg Loads Following Mass Drug Administration.
Vlaminck J, Supali T, Geldhof P, Hokke CH, Fischer PU, Weil GJ.
PLoS Negl Trop Dis. 2016 Mar 18;10(3):e0004532. doi: 10.1371/journal.pntd.0004532. eCollection 2016 Mar.

Novel O-linked methylated glycan antigens decorate secreted immunodominant glycoproteins from the intestinal nematode Heligmosomoides polygyrus.
Hewitson JP, Linh Nguyen D, van Diepen A, Smit CH, Koeleman CA, McSorley HJ, Murray J, Maizels RM, Hokke CH.
Int J Parasitol. 2016 Mar;46(3):157-70.

Local Antiglycan Antibody Responses to Skin Stage and Migratory Schistosomula of Schistosoma japonicum.
Smit CH, Kies CL, McWilliam HE, Meeusen EN, Hokke CH, van Diepen A.
Infect Immun. 2015 Oct 12;84(1):21-33. doi: 10.1128/IAI.00954-15.

Surface expression patterns of defined glycan antigens change during Schistosoma mansoni cercarial transformation and development of schistosomula.
Smit CH, Homann A, van Hensbergen VP, Schramm G, Haas H, van Diepen A, Hokke CH.
Glycobiology. 2015 Dec;25(12):1465-79. doi: 10.1093/glycob/cwv066. Epub 2015 Sep 7.

The N-glycan on Asn54 affects the atypical N-glycan composition of plant-produced interleukin-22, but does not influence its activity.
Wilbers RH, Westerhof LB, Reuter LJ, Castilho A, van Raaij DR, Nguyen DL, Lozano-Torres JL, Smant G, Hokke CH, Bakker J, Schots A.
Plant Biotechnol J. 2015 Jun 8. doi: 10.1111/pbi.12414. [Epub ahead of print]

Glycomic analysis of life stages of the human parasite Schistosoma mansoni reveals developmental expression profiles of functional and antigenic glycan motifs.
Smit CH, van Diepen A, Nguyen DL, Wuhrer M, Hoffmann KF, Deelder AM, Hokke CH.
Mol Cell Proteomics. 2015 Jul;14(7):1750-69. Epub 2015 Apr 16.

Development of a Schistosoma mansoni shotgun O-glycan microarray and application to the discovery of new antigenic schistosome glycan motifs.
van Diepen A, van der Plas AJ, Kozak RP, Royle L, Dunne DW, Hokke CH.
Int J Parasitol. 2015 Jun;45(7):465-75. doi: 10.1016/j.ijpara.2015.02.008. Epub 2015 Mar 26.

Drug-Induced Exposure of Schistosoma mansoni Antigens SmCD59a and SmKK7.
Reimers N, Homann A, Höschler B, Langhans K, Wilson RA, Pierrot C, Khalife J, Grevelding CG, Chalmers IW, Yazdanbakhsh M, Hoffmann KF, Hokke CH, Haas H, Schramm G.
PLoS Negl Trop Dis. 2015 Mar 16;9(3):e0003593. doi: 10.1371/journal.pntd.0003593. eCollection 2015 Mar.

Synthesis and microarray-assisted binding studies of core xylose and fucose containing N-glycans.
Brzezicka K, Echeverria B, Serna S, van Diepen A, Hokke CH, Reichardt NC.
ACS Chem Biol. 2015 May 15;10(5):1290-302. doi: 10.1021/cb501023u. Epub 2015 Feb 23.

Excreted/secreted Schistosoma mansoni venom allergen-like 9 (SmVAL9) modulates host extracellular matrix remodelling gene expression.
Yoshino TP, Brown M, Wu XJ, Jackson CJ, Ocadiz-Ruiz R, Chalmers IW, Kolb M, Hokke CH, Hoffmann KF.
Int J Parasitol. 2014 May 21. [Epub ahead of print]

Binding of von Willebrand Factor and plasma proteins to the eggshell of Schistosoma mansoni.
Dewalick S, Hensbergen PJ, Bexkens ML, Grosserichter-Wagener C, Hokke CH, Deelder AM, de Groot PG, Tielens AG, van Hellemond JJ.
Int J Parasitol. 2014 Feb 18. pii: S0020-7519(14)00032-0.  [Epub ahead of print]

In-depth proteomic and glycomic analysis of the adult-stage Cooperia oncophora excretome/secretome.
Borloo J, De Graef J, Peelaers I, Nguyen DL, Mitreva M, Devreese B, Hokke CH, Vercruysse J, Claerebout E, Geldhof P.
J Proteome Res. 2013 Sep 6;12(9):3900-11. doi: 10.1021/pr400114y. Epub 2013 Aug 20.

Specific glycan elements determine differential binding of individual egg glycoproteins of the human parasite Schistosoma mansoni by host C-type lectin receptors.
Meevissen MH, Driessen NN, Smits HH, Versteegh R, van Vliet SJ, van Kooyk Y, Schramm G, Deelder AM, Haas H, Yazdanbakhsh M, Hokke CH.
Int J Parasitol. 2012;42(3):269-77.

Circulating Biomphalaria glabrata hemocyte subpopulations possess shared schistosome glycans and receptors capable of binding larval glycoconjugates.
Yoshino TP, Wu XJ, Gonzalez LA, Hokke CH.
Exp Parasitol. 2012 Oct 18. pii: S0014-4894(12)00313-X. doi: 10.1016/j.exppara.2012.10.002. [Epub ahead of print]

Glycan-dependent immunogenicity of recombinant soluble trimeric hemagglutinin.
de Vries RP, Smit CH, de Bruin E, Rigter A, de Vries E, Cornelissen LA, Eggink D, Chung NP, Moore JP, Sanders RW, Hokke CH, Koopmans M, Rottier PJ, de Haan CA.
J Virol. 2012 Nov;86(21):11735-44. doi: 10.1128/JVI.01084-12. Epub 2012 Aug 22.

Differential anti-glycan antibody responses in Schistosoma mansoni-infected children and adults studied by shotgun glycan microarray.
van Diepen A, Smit CH, van Egmond L, Kabatereine NB, Pinot de Moira A, Dunne DW, Hokke CH.
PLoS Negl Trop Dis. 2012;6(11):e1922.Epub 2012 Nov 29.

Schistosome-derived omega-1 drives Th2 polarization by suppressing protein synthesis following internalization by the mannose receptor.
Everts B, Hussaarts L, Driessen NN, Meevissen MH, Schramm G, van der Ham AJ, van der Hoeven B, Scholzen T, Burgdorf S, Mohrs M, Pearce EJ, Hokke CH, Haas H, Smits HH, Yazdanbakhsh M.
J Exp Med. 2012 Sep 24;209(10):1753-67, S1. Epub 2012 Sep 10.

Glycan-dependent immunogenicity of recombinant soluble trimeric hemagglutinin.
de Vries RP, Smit CH, de Bruin E, Rigter A, de Vries E, Cornelissen LA, Eggink D, Chung NP, Moore JP, Sanders RW, Hokke CH, Koopmans M, Rottier PJ, de Haan CA.
J Virol. 2012 Aug 22. [Epub ahead of print]

Glycotope Sharing between Snail Hemolymph and Larval Schistosomes: Larval Transformation Products Alter Shared Glycan Patterns of Plasma Proteins.
Yoshino TP, Wu XJ, Liu H, Gonzalez LA, Deelder AM, Hokke CH.
PLoS Negl Trop Dis. 2012 Mar;6(3):e1569.

Parasite glycans and antibody-mediated immune responses in Schistosoma infection.
Van Diepen A, van der Velden NS, Smit CH, Meevissen MH, Hokke CH.
Parasitology. 2012 Mar 16:1-12.

Changes in antigen-specific IgG1 Fc N-glycosylation upon influenza and tetanus vaccination.
Selman MH, de Jong SE, Soonawala D, Kroon FP, Adegnika AA, Deelder AM, Hokke CH, Yazdanbakhsh M, Wuhrer M.
Mol Cell Proteomics. 2012 Apr;11(4):M111.014563. Epub 2011 Dec 19.

Schistosoma mansoni egg glycoproteins and C-type lectins of host immune cells: Molecular partners that shape immune responses.
Meevissen MH, Yazdanbakhsh M, Hokke CH.
Exp Parasitol. 2012 Sep;132(1):14-21.

Targeted glycoproteomic analysis reveals that kappa-5 is a major, uniquely glycosylated component of Schistosoma mansoni egg antigens.
Meevissen MH, Balog CI, Koeleman CA, Doenhoff MJ, Schramm G, Haas H, Deelder AM, Wuhrer M, Hokke CH.
Mol Cell Proteomics. 2011 May;10(5):M110.005710

The proteome of the insoluble Schistosoma mansoni eggshell skeleton.
Dewalick S, Bexkens ML, van Balkom BW, Wu YP, Smit CH, Hokke CH, de Groot PG, Heck AJ, Tielens AG, van Hellemond JJ.
Int J Parasitol. 2011 Apr; 41(5):523-32.

Ligand identification of carbohydrate-binding proteins employing a biotinylated glycan binding assay and tandem mass spectrometry.
Wuhrer M, van Remoortere A, Balog CI, Deelder AM, Hokke CH.
Anal Biochem. 2010 Nov 15;406(2):132-40.

Helminths and dendritic cells: sensing and regulating via pattern recognition receptors, Th2 and Treg responses.
Everts B, Smits HH, Hokke CH, Yazdanbakhsh M.
Eur J Immunol. 2010 Jun;40(6):1525-37.

Lack of complex N-glycans on HIV-1 envelope glycoproteins preserves protein conformation and entry function.
Eggink D, Melchers M, Wuhrer M, van Montfort T, Dey AK, Naaijkens BA, David KB, Le Douce V, Deelder AM, Kang K, Olson WC, Berkhout B, Hokke CH, Moore JP, Sanders RW.
Virology. 2010 Jun 5;401(2):236-47.

Structural Characterization of Glycans on Omega-1, a Major Schistosoma mansoni Egg Glycoprotein That Drives Th2 Responses.
Meevissen MH, Wuhrer M, Doenhoff MJ, Schramm G, Haas H, Deelder AM, Hokke CH.
J Proteome Res. 2010 May 7;9(5):2630-42.

Mass spectrometric identification of aberrantly glycosylated human apolipoprotein C-III peptides in urine from Schistosoma mansoni-infected individuals.
Balog CI, Mayboroda OM, Wuhrer M, Hokke CH, Deelder AM, Hensbergen PJ.
Mol Cell Proteomics. 2010 Apr;9(4):667-81.

Omega-1, a glycoprotein secreted by Schistosoma mansoni eggs, drives Th2 responses.
Everts B, Perona-Wright G, Smits HH, Hokke CH, van der Ham AJ, Fitzsimmons CM, Doenhoff MJ, van der Bosch J, Mohrs K, Haas H, Mohrs M, Yazdanbakhsh M, Schramm G.
J Exp Med. 2009 Aug 3;206(8):1673-80.

Glycotope analysis in miracidia and primary sporocysts of Schistosoma mansoni: differential expression during the miracidium-to-sporocyst transformation.
Peterson NA, Hokke CH, Deelder AM, Yoshino TP.
Int J Parasitol. 2009 Oct;39(12):1331-44.

Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs.
Schramm G, Hamilton JV, Balog CI, Wuhrer M, Gronow A, Beckmann S, Wippersteg V, Grevelding CG, Goldmann T, Weber E, Brattig NW, Deelder AM, Dunne DW, Hokke CH, Haas H, Doenhoff MJ.
Mol Biochem Parasitol. 2009 Jul;166(1):4-14.

Characterization of a diagnostic Fab fragment binding trimeric Lewis X.
de Geus DC, van Roon AM, Thomassen EA, Hokke CH, Deelder AM, Abrahams JP.
Proteins. 2009 Aug 1;76(2):439-47.

Molecular and functional characterization of a tandem-repeat galectin from the freshwater snail Biomphalaria glabrata, intermediate host of the human blood fluke Schistosoma mansoni.
Yoshino TP, Dinguirard N, Kunert J, Hokke CH.
Gene. 2008 Mar 31;411(1-2):46-58.

Serum antibody screening by surface plasmon resonance using a natural glycan microarray.
de Boer AR, Hokke CH, Deelder AM, Wuhrer M.
Glycoconj J. 2008 Jan;25(1):75-84.

Mass spectrometric detection of urinary oligosaccharides as markers of Schistosoma mansoni infection.
Robijn ML, Planken J, Kornelis D, Hokke CH, Deelder AM.
Trans R Soc Trop Med Hyg. 2008 Jan;102(1):79-83.

General microarray technique for immobilization and screening of natural glycans.
de Boer AR, Hokke CH, Deelder AM, Wuhrer M.
Anal Chem. 2007 Nov 1;79(21):8107-13.

Glycomics-driven discoveries in schistosome research.
Hokke CH, Deelder AM, Hoffmann KF, Wuhrer M.
Exp Parasitol. 2007 Nov;117(3):275-83.. Review.

Integrating transcriptome, proteome and glycome analyses of Schistosoma biology.
Hokke CH, Fitzpatrick JM, Hoffmann KF.
Trends Parasitol. 2007 Apr;23(4):165-74. Review.

Targeted identification of a unique glycan epitope of Schistosoma mansoni egg antigens using a diagnostic antibody.
Robijn ML, Koeleman CA, Wuhrer M, Royle L, Geyer R, Dwek RA, Rudd PM, Deelder AM, Hokke CH.
Mol Biochem Parasitol. 2007 Feb;151(2):148-61

Schistosoma mansoni eggs excrete specific free oligosaccharides that are detectable in the urine of the human host.
Robijn ML, Koeleman CA, Hokke CH, Deelder AM.
Mol Biochem Parasitol. 2007 Feb;151(2):162-72. 

Efficient introduction of a bisecting GlcNAc residue in tobacco N-glycans by expression of the gene encoding human N-acetylglucosaminyltransferase III.
Rouwendal GJ, Wuhrer M, Florack DE, Koeleman CA, Deelder AM, Bakker H, Stoopen GM, van Die I, Helsper JP, Hokke CH, Bosch D.
Glycobiology. 2007 Mar;17(3):334-44. Epub 2006 Dec 19.

The host's genetic background determines the extent of angiogenesis induced by schistosome egg antigens.
Van de Vijver KK, Colpaert CG, Jacobs W, Kuypers K, Hokke CH, Deelder AM, Van Marck EA.
Acta Trop. 2006 Oct;99(2-3):243-51.

Gender-specific expression of complex-type N-glycans in schistosomes.
Wuhrer M, Koeleman CA, Fitzpatrick JM, Hoffmann KF, Deelder AM, Hokke CH.
Glycobiology. 2006 Oct;16(10):991-1006.

IPSE/alpha-1, a major secretory glycoprotein antigen from schistosome eggs, expresses the Lewis X motif on core-difucosylated N-glycans.
Wuhrer M, Balog CI, Catalina MI, Jones FM, Schramm G, Haas H, Doenhoff MJ, Dunne DW, Deelder AM, Hokke CH.
FEBS J. 2006 May;273(10):2276-92.

Repeats of LacdiNAc and fucosylated LacdiNAc on N-glycans of the human parasite Schistosoma mansoni.
Wuhrer M, Koeleman CA, Deelder AM, Hokke CH.
FEBS J. 2006 Jan;273(2):347-61.

LacdiNAc- and LacNAc-containing glycans induce granulomas in an in vivo model for schistosome egg-induced hepatic granuloma formation.
Van de Vijver KK, Deelder AM, Jacobs W, Van Marck EA, Hokke CH.
Glycobiology. 2006 Mar;16(3):237-43.  

Collaborative glycomics publications

IgG1 Fc N-glycan galactosylation as a biomarker for immune activation.de Jong SE, Selman MH, Adegnika AA, Amoah AS, van Riet E, Kruize YC, Raynes JG, Rodriguez A, Boakye D, von Mutius E, Knulst AC, Genuneit J, Cooper PJ, Hokke CH, Wuhrer M, Yazdanbakhsh M.Sci Rep. 2016 Jun 16;6:28207.

Protein and small non-coding RNA-enriched extracellular vesicles are released by the pathogenic blood fluke Schistosoma mansoni.Nowacki FC, Swain MT, Klychnikov OI, Niazi U, Ivens A, Quintana JF, Hensbergen PJ, Hokke CH, Buck AH, Hoffmann KF.J Extracell Vesicles. 2015 Oct 5;4:28665. doi: 10.3402/jev.v4.28665. eCollection 2015.

The N-glycan on Asn54 affects the atypical N-glycan composition of plant-produced interleukin-22, but does not influence its activity.
Wilbers RH, Westerhof LB, Reuter LJ, Castilho A, van Raaij DR, Nguyen DL, Lozano-Torres JL, Smant G, Hokke CH, Bakker J, Schots A.
2015 Jun 8. doi: 10.1111/pbi.12414. [Epub ahead of print]Plant Biotechnol J

N-glycomic profiling as a tool to separate rectal adenomas from carcinomas.
Kaprio T, Satomaa T, Heiskanen A, Hokke CH, Deelder AM, Mustonen H, Hagström J, Carpen O, Saarinen J, Haglund C.
Mol Cell Proteomics. 2014 Dec 1. [Epub ahead of print]

Monomeric IgA can be produced in planta as efficient as IgG, yet receives different N-glycans.Westerhof LB, Wilbers RH, van Raaij DR, Nguyen DL, Goverse A, Henquet MG, Hokke CH, Bosch D, Bakker J, Schots A.Plant Biotechnol J. 2014 Sep 4. doi: 10.1111/pbi.12251. [Epub ahead of print]

Profiling glycosyltransferase activities by tritium imaging of glycan microarrays.
Serna S, Hokke CH, Weissenborn M, Flitsch S, Martin-Lomas M, Reichardt NC.
Chembiochem. 2013 May 10;14(7):862-9. Epub 2013 Apr 8.

Plasma protein N-glycan profiles are associated with calendar age, familial longevity and health.
Ruhaak LR, Uh HW, Beekman M, Hokke CH, Westendorp RG, Houwing-Duistermaat JJ, Wuhrer M, Deelder AM, Slagboom PE.
J Proteome Res. 2011 Apr 1;10(4):1667-74.

Decreased levels of bisecting GlcNAc glycoforms of IgG are associated with human longevity.
Ruhaak LR, Uh HW, Beekman M, Koeleman CA, Hokke CH, Westendorp RG, Wuhrer M, Houwing-Duistermaat JJ, Slagboom PE, Deelder AM.
PLoS One. 2010 Sep 7;5(9):e12566.

Golgi targeting of Drosophila melanogaster beta4GalNAcTB requires a DHHC protein family-related protein as a pilot.
Johswich A, Kraft B, Wuhrer M, Berger M, Deelder AM, Hokke CH, Gerardy-Schahn R, Bakker H.
J Cell Biol. 2009 Jan 12;184(1):173-83.

Hydrophilic interaction chromatography-based high-throughput sample preparation method for N-glycan analysis from total human plasma glycoproteins.
Ruhaak LR, Huhn C, Waterreus WJ, de Boer AR, Neusüss C, Hokke CH, Deelder AM, Wuhrer M.
Anal Chem. 2008 Aug 1;80(15):6119-26.

Glycosylation profiling of immunoglobulin G (IgG) subclasses from human serum.
Wuhrer M, Stam JC, van de Geijn FE, Koeleman CA, Verrips CT, Dolhain RJ, Hokke CH, Deelder AM.
Proteomics. 2007 Nov;7(22):4070-81.

Distinct contributions of beta 4GalNAcTA and beta 4GalNAcTB to Drosophila glycosphingolipid biosynthesis.
Stolz A, Haines N, Pich A, Irvine KD, Hokke CH, Deelder AM, Gerardy-Schahn R, Wuhrer M, Bakker H.
Glycoconj J. 2008 Feb;25(2):167-75.

Glycoproteomics based on tandem mass spectrometry of glycopeptides.
Wuhrer M, Catalina MI, Deelder AM, Hokke CH.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15;849(1-2):115-28. Review.

Mass spectrometry of proton adducts of fucosylated N-glycans: fucose transfer between antennae gives rise to misleading fragments.
Wuhrer M, Koeleman CA, Hokke CH, Deelder AM.
Rapid Commun Mass Spectrom. 2006;20(11):1747-54.

Oligosaccharide analysis by capillary-scale high-pH anion-exchange chromatography with on-line ion-trap mass spectrometry.
Bruggink C, Wuhrer M, Koeleman CA, Barreto V, Liu Y, Pohl C, Ingendoh A, Hokke CH, Deelder AM. J
Chromatogr B Analyt Technol Biomed Life Sci. 2005 Dec 27;829(1-2):136-43.