PacBio Revio
The LGTC installed the PacBio Revio platform in March 2024. The Revio platform offers affordable, high-throughput, highly accurate long read sequencing.
Technology
The Revio is an upgrade of the Sequel II platform. The high-density Single Molecule Real Time (SMRT) cells contain 25 million zero-mode waveguides (ZMWs) capable of sequencing 90Gb+ per SMRT cell.
Each time a nucleotide is incorporated within a ZMW, it is held in place by the polymerase and a fluorescent signal is detected in real time. The incorporated nucleotides are identified by four different fluorescent labels corresponding to A, T, G, and C. The collection of data consists of the recording of a movie of light pulses (pulse data) which is then translated into bases (base data). More information about the technology can be found on https://www.pacb.com.
Applications
- De novo assembly of small and large genomes with 99.95% accuracy (Q33)
- Sequence full-length transcriptomes or targeted transcripts
- High accuracy variant calling for all variant types
- Target regions not currently accessible by other technologies
- Detect genomic variation in complex populations on all size scales
- Detect epigenetic modification including polymerase kinetics
