Peptide Facility (cell and chemical biology)

Equipment

The Peptide is both an organic chemistry laboratory, as well as a biochemistry and chemical biology laboratory. We work with reagents - ranging from in-house prepared small molecule inhibitor libraries up to activity-based probes - consisting of modified di-ubiquitin proteins. Therefore, we routinely use a wide variety of equipment.

Peptide synthesizers

  • MultiSynTech Syro II - 1 x 24 parallel synthesizer (each 40 umol)
  • MultiSynTech Syro II - 6 x 96 parallel synthesizer (each 4 umol)
  • Intavis MultiPep CF 

Mass Spectrometers

  • WATERS Alliance  LCMS coupled to a LCT-premier Mass Spectrometer
  • WATERS Acquity H-class UPLC coupled to a high resolution XEVO G2S-XTOF Mass Spectrometer 

HPLC-systems

  • WATERS 2545 LCMS coupled to a 3100 Mass Spectrometer; mass triggered collection during HPLC purification mostly used for small molecules
  • WATERS 2535 LCMS coupled to a 2545 UV-VIS detector; routinely used for large scale purifications of Ubl's 
  • WATERS 1525 EF LCMS coupled to a 2487 dual labda Absorbance Detector; routinely used for peptide purifications
  • Shimadzu LC-20 LCMS system; routinely used for small scale purifications of peptides and Ubl's

FPLC-systems

  • 2x BUCHI-C620 automated column set-ups for straight phase silica separations
  • BIORAD NGC system for cat-/anion exchange and size exclusion gel filtration

NMR-system

  • Bruker Ultrashield 300 MHz