Single Cell Sequencing

10x Genomics Chromium X platform

The Leiden Genome Technology Center acquired the 10x Genomics Chromium X platform in January 2022

The market leader for single-cell studies, the Chromium controller series is an advanced microfluidics platform that can process up to 16 samples at once, generating up to 1 million barcoded single-cells per run. More information about the technology can be found on 10xgenomics.com.

Single-cell 3’ solution
High-throughput single-cell expression measurements that enable the discovery of gene expression dynamics and molecular profiling of individual cell types

Single cell V(D)J solution
A comprehensive, scalable tool for profiling full-length paired V(D)J transcripts from hundreds to millions of lymphocytes.  This new solution enables the assembly of full-length V(D)J sequences on a cell-by-cell basis, providing high-resolution insights into the adaptive immune system.

Single cell ATAC-seq solution
Reveal the epigenomic landscape and analyse chromatin accessibility cell-by-cell to decipher gene regulatory mechanisms.

Single cell multiome solution
Explore combined gene expression and chromatin accessibility data from the same cell to uncover gene regulatory programs

Request a quote via info@lgtc.nl

Dolomite Bio Nadia

The Nadia Instrument is a fully automated system to encapsulate samples. This microfluidic droplet-based platform can be used for single-cell capture. It’s possible to encapsulate up to 8 samples, in parallel, in under 20 mins. Over 50,000 single cells can be captured per cartridge in a run. More information about the technology can be found on dolomite-bio.com.

Applications

• Single-cell RNA-Seq
• Single-nucleus RNA-Seq
• Encapsulation of cells in agarose

Formulatrix MANTIS Liquid Handler

The MANTIS dispenses any reagent efficiently and reproducibly regardless of volume, viscosity, temperature, or concentration.  It accurately dispenses a range of volumes from 0.1ul – 200ul in formats from 6-well to 1536-well plates.  Different wells can receive different volumes and different reagents, which allows for a variety of applications including reaction miniaturization, plate dispensing, and plate normalization. More information about the technology can be found on formulatrix.com.

Applications

• Assay Development and Design of Experiment
• Next-Generation Sequencing – Library Preparation
• Polymerase Chain-reaction Workflows – Real-time and Digital
• Enzyme-Linked Immunosorbent Assays
• Cell Culture and Bead-Based Assays