The LUMC human iPSC Hotel: Generation of research grade human iPSCs and beyond

Reprogramming vectors

  • Polycistronic lentivirus: Integrates into the host genome and works efficiently for reprogramming of most somatic cell types. hiPSCs derived from fibroblasts usually have maximally 2 proviral integrations (no information available for other cell types). Transgenes can be excised using the FLP enzyme. The LUMC hiPSC core facility service does not include screening of proviral integrations (sites or numbers) or excision of transgenes on a standard basis. It is possible to obtain a license for commercial use.
  • Episomal plasmids: In principle non-integrating but (partial) integrations into the host genome have been reported for a number of hiPSC lines. The LUMC hiPSC core facility service does not include screening for the absence of (partial) integrations.  It is possible to obtain a license for commercial use. 
  • Sendai virus (SeV): Non-integrating. The LUMC hiPSC core facility has a limited capacity for reprogramming with the SeV-based vector system. SeV-hiPSCs need to be maintained at the  ML-2 safety level at all times unless absence of SeV has been confirmed by RT-PCR and IF staining. The LUMC hiPSC core facility checks for the absence of SeV in the 3 clones characterized and delivered at the passage indicated to the end user. Customers are responsible for testing for the absence of SeV if lines are provided at lower passage than included in standard testing or other clones are used. Protocols for SeV detection are available upon request. hiPSCs generated with SeV are excluded from commercial use. 
  • Synthetic RNA: Non-integrating. Not applicable for blood reprogramming. It is possible to obtain a license for commercial use 

Reprogramming