Center for Microbiome Analyses and Therapeutics

Our dedicated and (BROK-)certified researchers and physicians have the know-how in the microbiome field to support your research projects. CMAT facilitates all aspects of microbiome research:

Study design

CMAT offers advice on local, national and international study design, subjects, sample size, sampling methods and storage conditions.

Sampling kits

We have the infrastructure in place to provide customized sampling kits for your research project. The sampling kits are ready-to-use and include instructions and materials for sampling and (room temperature or cooled) transport.

Sample processing

Samples can be aliquoted for DNA- and/or culture-based methods. DNA extraction will be performed according to our standard operating procedures, which are optimized for feces and for low biomass samples such as skin swabs and urine. We concentrate our efforts on quality control during DNA extraction and sequencing.

Sample storage

Storage during and after the experiment are of utmost importance for high quality data and reusability. As such, CMAT advises on making aliquots for DNA- and/or culture-based methods to make your research future-proof.

Next generation sequencing

CMAT collaborates with shared facilities of the Leiden Bio Science Park, such as GenomeScan. We will be the intermediary for your research project. The microbiome sequencing options include:

• 16S rRNA gene sequencing
• ITS2 sequencing
• Shotgun metagenomic sequencing

Bioinformatic support

Microbiome sequencing data generates intricate data. We have the bioinformatic skills and infrastructure in place to help you answer your research questions. Moreover, CMAT makes sequencing data accessible and stimulates re-use of previously collected data.

Support for grant applications

Have you not found what you are looking for? Please contact us to discuss options.