- (Innate) Immunology
- Cell kinetics, maturation & differentiation
- Single-cell analysis
Tamar Tak is a researcher in the department of parasitology working for Dr. Hermelijn Smit.
His work is focused on obtaining and analyzing high-dimensional data on white blood cells from clinical samples. To reach this goal he uses state-of-the-art techniques such as single-cell culture systems, single-cell RNA sequencing and Mass/Flow Cytometry.
Tamar Tak obtained his BSc in biomedical sciences and his MSc in Immunity and Infection at Utrecht University. During his studies he conducted research internships at the UMC Utrecht and at the Westmead Institute in Sydney, Australia.
He continued his PhD at the UMC Utrecht at the Laboratory of Translational Immunology and department of Respiratory Medicine with Prof. Leo Koenderman. There he aimed to determine the lifespans and differentiation/maturation kinetics of innate immune cells in the blood of humans under homeostatic conditions as well as in non-homeostatic conditions including asthma, cystic fibrosis and in a human experimental endotoxemia model. Furthermore, by the use of proteomics he was able to propose the immune-suppressive hypersegmented neutrophil as a separately developing neutrophil subset that is only recruited to the blood in inflammatory conditions.
After obtaining his PhD cum laude in 2016, he moved to the department of Physical Chemistry at Institut Curie in Paris to further study the where he employed cellular barcoding to further study the development of white blood cells and where he developed high-throughput methods for single-cell cultures and its subsequent analysis.
All Tamar’s projects have relied on technical innovation and on close collaborations with clinicians as well as mathematicians and bioinformaticians. He is currently applying and further extending these skills in the LUMC.
A complete and up-to-date list of publications can be found on: https://orcid.org/0000-0002-5959-7927
Tak T, Prevedello G, Simon G, Paillon N, Duffy KR, Perié L: Simultaneous tracking of division and differentiation from individual hematopoietic stem and progenitor cells reveals within-family homogeneity despite population heterogeneity. BioRxiv 2019:586354.
Tak T, Rygiel TP, Karnam G, Bastian OW, Boon L, Viveen M, Coenjaerts FE, Meyaard L, Koenderman L, Pillay J: Neutrophil-mediated Suppression of Influenza-induced Pathology Requires CD11b/CD18 (MAC-1). Am J Respir Cell Mol Biol 2018, 58(4):492-499.
Tak T, Drylewicz J, Conemans L, de Boer RJ, Koenderman L, Borghans JAM, Tesselaar K: Circulatory and maturation kinetics of human monocyte subsets in vivo. Blood 2017, 130(12):1474-1477.
Tak T, Wijten P, Heeres M, Pickkers P, Scholten A, Heck AJR, Vrisekoop N, Leenen LP, Borghans JAM, Tesselaar K et al: Human CD62L(dim) neutrophils identified as a separate subset by proteome profiling and in vivo pulse-chase labeling. Blood 2017, 129(26):3476-3485.
Tak T, van Groenendael R, Pickkers P, Koenderman L: Monocyte Subsets Are Differentially Lost from the Circulation during Acute Inflammation Induced by Human Experimental Endotoxemia. J Innate Immun 2017, 9(5):464-474.
Tak T, Hilvering B, Tesselaar K, Koenderman L: Similar activation state of neutrophils in sputum of asthma patients irrespective of sputum eosinophilia. Clin Exp Immunol 2015, 182(2):204-212.
Ter Haar NM, Tak T, Mokry M, Scholman RC, Meerding JM, de Jager W, Verwoerd A, Foell D, Vogl T, Roth J et al: Reversal of Sepsis-Like Features of Neutrophils by Interleukin-1 Blockade in Patients With Systemic-Onset Juvenile Idiopathic Arthritis. Arthritis Rheumatol 2018, 70(6):943-956.
Pillay J, Tak T, Kamp VM, Koenderman L: Immune suppression by neutrophils and granulocytic myeloid-derived suppressor cells: similarities and differences. Cell Mol Life Sci 2013, 70(20):3813-3827.
Tak T, Tesselaar K, Pillay J, Borghans JA, Koenderman L: What's your age again? Determination of human neutrophil half-lives revisited. J Leukoc Biol 2013, 94(4):595-601
Leids Universitair Medisch Centrum
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