Dr. Christian Freund: Generation of human pluripotent stem cells (hiPSCs) for in vitro applications

Principal Investigator:  Dr. Christian Freund,  Dr. Harald Mikkers

Researcher scientists:  Dr. Karine Raymond, Dr. Umesh Chaudhari

Research technician:   Marga Bouma, Merve Tok

The LUMC hiPSC core facility generates research grade human induced pluripotent stem cells (hiPSCs) with state of the art techniques for LUMC researchers and external parties. Since 2010 more than 130 hiPSC lines have been generated from somatic tissues such as skin, blood, urine, milk teeth with integrating vectors (lentivirus) and non-integrating episomal plasmids, Sendai virus and synthetic RNA. Pluripotency of hiPSCs is assessed by immunofluorescent staining of pluripotency markers in undifferentiated cells as well as of markers for ectoderm, mesoderm and endoderm after spontaneous differentiation. In a yearly training course the LUMC hiPSC core facility teaches maintenance of undifferentiated cells, freezing, thawing as well as directed differentiation into various cell types. Furthermore the LUMC hiPSC core facility supports project planning, provides control hiPSCs and protocols for differentiation of hiPSCs into cardiovascular cells. 

More detailed information can be found on the LUMC hiPSC core facility page.